Targeted O2 delivery by blood substitutes: in vitro arteriolar simulations of first- and second-generation products
Targeted O2 delivery by blood substitutes: in vitro arteriolar simulations of first- and second-generation products
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DOI：
10.1016/j.mvr.2008.07.002
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发表时间：
2008-11-01
影响因子：
3.1
通讯作者：
Winslow, Robert
中科院分区：
文献类型：
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作者：
Cole, Russell;Vandegriff, Kim;Winslow, Robert
从商业生产的血红蛋白为基础的O-2载体（HBOCs）和红细胞（RBC）流经动脉大小（25 μ m）的管道的混合物的O-2运输进行了模拟。一个广义的治疗外的O-2运输过程是用来反映生理条件的变化，如增加O-2消耗。在所考虑的HBOC中，聚合牛血红蛋白（PolyBvHb，p50 = 54 mmHg）、四聚体交联人血红蛋白（α α Hb，p50 = 33 mmHg）和聚乙二醇化人血红蛋白（MP4，p50 = 5 mmHg），与单独的RBC悬浮液相比，只有MP4不增加O-2提取率。小动脉O-2提取的减少可能通过防止O-2诱导的血管活性和最大化毛细血管可用的O-2供应而对HBOCs有益。来自体内HBOC输注实验的结果不能通过模型预测，除非与MP4相比，PolyBvHb具有显著降低的腔外O-2转运阻力。这一结果与文献中显示小动脉O-2消耗量随血管内pO（2）增加的结果一致。(C)2008年爱思唯尔公司版权所有
The O-2 transport from mixtures of commercially produced hemoglobin-based O-2 carriers (HBOCs) and red blood cells (RBCs) flowing through arteriolar-sized (25-mu m) conduits is simulated. A generalized treatment of extraluminal O-2 transport processes is used to reflect variations in physiological conditions, such as increased O-2 consumption. Of the HBOCs considered, polymerized bovine hemoglobin (PolyBvHb, p50 = 54mmHg), tetrameric cross-linked human hemoglobin (alpha alpha Hb, p50 = 33mmHg), and PEGylated human hemoglobin (MP4, p50 = 5mmHg), only MP4 does not increase O-2 extraction ratios when compared to RBC suspensions alone. A reduction in arteriolar O-2 extraction is likely to be beneficial for HBOCs by preventing O-2-induced vasoactivity and maximizing the Supply of O-2 available to the capillaries. Results from in vivo HBOC transfusion experiments cannot be predicted by the model, unless PolyBvHb has a significant decrease in extraluminal O-2 transport resistance as compared to MP4. This result is consistent with the literature that shows arteriolar O-2 consumption to increase with intravascular pO(2). (C) 2008 Elsevier Inc. All rights reserved