In Vitro Management of Hospital Pseudomonas aeruginosa Biofilm Using Indigenous T7-Like Lytic Phage
In Vitro Management of Hospital Pseudomonas aeruginosa Biofilm Using Indigenous T7-Like Lytic Phage
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DOI：
10.1007/s00284-010-9710-6
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发表时间：
2011-02-01
影响因子：
2.6
通讯作者：
Kapadnis, Balasaheb
中科院分区：
文献类型：
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作者：
Ahiwale, Sangeeta;Tamboli, Nilofer;Kapadnis, Balasaheb
铜绿假单胞菌是一种能够形成生物被膜并污染医疗环境的人类病原体，在世界各地的医院中占65%的死亡率。本研究是为了分离抗生物膜形成Ps的裂解酶。铜绿假单胞菌医院分离株，并将它们用于微量滴定板中生物膜的体外管理。多药耐药的Ps。铜绿假单胞菌通过标准微生物方法从马哈拉施特拉邦的Pimpri-Chinchwad及其周围的医院环境中分离。用双层琼脂平板法从帕瓦纳河水中分离到对这些菌株具有较强杀伤力的溶菌。一种宿主范围广的噬菌体细菌病毒Ps。选择铜绿假单胞菌噬菌体（BVPaP-3）。电子显微镜显示BVPaP-3噬菌体是T7样噬菌体，并且是噬菌体种类gh-1的亲戚。在MOI-0.001的噬菌体可以阻止Ps形成生物膜。铜绿假单胞菌医院菌株-6（HS 6）。它还可以在24小时内分散钉上所有医院分离株（HS 1-HS 6）的预形成生物膜。通过监测在PEG上以及在孔中的各个细菌和噬菌体的cfu的对数百分比减少和pfu的对数百分比增加来研究生物膜的分散。扫描电子显微镜证实噬菌体BVPaP-3确实导致生物膜减少和细菌细胞杀伤。实验室研究证明BVPaP-3是一种高效的预防和驱散Ps生物膜的噬菌体。铜绿。噬菌体BVPaP-3可作为生物消毒剂用于控制医疗器械中的生物膜问题。
Pseudomonas aeruginosa, a human pathogen capable of forming biofilm and contaminating medical settings, is responsible for 65% mortality in the hospitals all over the world. This study was undertaken to isolate lytic phages against biofilm forming Ps. aeruginosa hospital isolates and to use them for in vitro management of biofilms in the microtiter plate. Multidrug resistant strains of Ps. aeruginosa were isolated from the hospital environment in and around Pimpri-Chinchwad, Maharashtra by standard microbiological methods. Lytic phages against these strains were isolated from the Pavana river water by double agar layer plaque assay method. A wide host range phage bacterial virus Ps. aeruginosa phage (BVPaP-3) was selected. Electron microscopy revealed that BVPaP-3 phage is a T7-like phage and is a relative of phage species gh-1. A phage at MOI-0.001 could prevent biofilm formation by Ps. aeruginosa hospital strain-6(HS6) on the pegs within 24 h. It could also disperse pre-formed biofilms of all hospital isolates (HS1-HS6) on the pegs within 24 h. Dispersion of biofilm was studied by monitoring log percent reduction in cfu and log percent increase in pfu of respective bacterium and phage on the peg as well as in the well. Scanning electron microscopy confirmed that phage BVPaP-3 indeed causes biofilm reduction and bacterial cell killing. Laboratory studies prove that BVPaP-3 is a highly efficient phage in preventing and dispersing biofilms of Ps. aeruginosa. Phage BVPaP-3 can be used as biological disinfectant to control biofilm problem in medical devices.