RNA-PROTEIN INTERACTIONS IN 30S RIBOSOMAL-SUBUNITS - FOLDING AND FUNCTION OF 16S RIBOSOMAL-RNA
RNA-PROTEIN INTERACTIONS IN 30S RIBOSOMAL-SUBUNITS - FOLDING AND FUNCTION OF 16S RIBOSOMAL-RNA
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DOI：
10.1126/science.2658053
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发表时间：
1989-05-19
期刊：
影响因子：
56.9
通讯作者：
NOLLER, HF
中科院分区：
文献类型：
--
作者：
STERN, S;POWERS, T;NOLLER, HF
在体外组装20个30s亚基核糖体蛋白的每一步中，化学探针方法都被用来“足迹”16rrna。这些实验提供了每个蛋白质相对于16SrRNA结构的位置信息，并为推导16SrRNA三维折叠的详细模型提供了基础。一些证据表明，蛋白质依赖的16SrRNA构象变化在核糖体组装的协同性以及30s亚基中16SrRNA构象和动力学的微调中起着重要作用。
Chemical probing methods have been used to "footprint" 16Sribosomal RNA (rRNA) at each step during the in vitro assembly of twenty 30Ssubunit ribosomal proteins. These experiments yield information about the location of each protein relative to the structure of 16SrRNA and provide the basis for derivation of a detailed model for the three-dimensional folding of 16SrRNA. Several lines of evidence suggest that protein-dependent conformational changes in 16SrRNA play an important part in the cooperativity of ribosome assembly and in fine-tuning of the conformation and dynamics of 16SrRNA in the 30Ssubunit.