In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression
In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression
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DOI：
10.1016/j.ymeth.2003.11.003
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发表时间：
2004-06-01
期刊：
影响因子：
4.8
通讯作者：
Teissié, J
中科院分区：
文献类型：
--
作者：
Golzio, M;Rols, MP;Teissié, J
电脉冲是成功用于在体外和体内将基因转移到活细胞中的非病毒方法之一。这种方法在基因和细胞治疗领域显示出前景。本文首先描述了控制小分子电透化的因素（
Electropulsation is one of the non-viral methods successfully used to transfer genes into living cells in vitro as in vivo. This approach shows promise in the field of gene and cellular therapies. The present paper first describes the factors controlling electropermeabilization to small molecules (