miR172 Regulates both Vegetative and Reproductive Development in the Perennial Woody Plant Jatropha curcas
miR172 Regulates both Vegetative and Reproductive Development in the Perennial Woody Plant Jatropha curcas
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miR172 调节多年生木本植物麻风树的营养和生殖发育。
DOI：
10.1093/pcp/pcy175
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发表时间：
2018-12-01
影响因子：
4.9
通讯作者：
Xu, Zeng-Fu
中科院分区：
文献类型：
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作者：
Tang, Mingyong;Bai, Xue;Xu, Zeng-Fu
麻疯树是一种很有前途的生物燃料生产原料，因为它的油非常适合加工生物喷气燃料和生物柴油。然而，麻疯树在亚热带地区表现出很长的幼虫期。MiR172是一种保守的非蛋白质编码的小RNA分子，有21个核苷酸，调节着广泛的发育过程。然而，到目前为止，还没有研究检查miR172在麻疯树中的功能。麻疯树中有5个miR172前体编码两个成熟的miR172，它们在所有组织中都有表达，其中在叶片中的表达水平最高，并且随着年龄的增加而上调。在转JcmiR172a基因的拟南芥和麻疯树中，过表达JcmiR172a导致了早花、畸形花和叶片形态的改变。在JcmiR172a过表达的转基因植株中，miR172靶基因的表达水平下调，而花识别基因的表达水平上调。有趣的是，我们发现JcmiR172可能通过调控纤维素和木质素生物合成基因的表达参与茎维管发育的调控。JcmiR172a的过表达促进了木质部的发育，抑制了韧皮部和髓的发育。这项研究有助于阐明miR172在多年生植物中的功能，miR172是一种已知的与年龄相关的miRNA，参与调节多年生植物的相变和器官发育。
Jatropha curcas is a promising feedstock for biofuel production because its oil is highly suitable for processing bio-jet fuels and biodiesel. However, Jatropha exhibits a long juvenile stage in subtropical areas. miR172, a conserved small non-protein-coding RNA molecule with 21 nucleotides, regulates a wide range of developmental processes. To date, however, no studies have examined the function of miR172 in Jatropha. There are five miR172 precursors encoding two mature miR172s in Jatropha, which are expressed in all tissues, with the highest expression level in leaves, and the levels are up-regulated with age. Overexpression of JcmiR172a resulted in early flowering, abnormal flowers, and altered leaf morphology in transgenic Arabidopsis and Jatropha. The expression levels of miR172 target genes were down-regulated, and the flower identity genes were up-regulated in the JcmiR172a-overexpressing transgenic plants. Interestingly, we showed that JcmiR172 might be involved in regulation of stem vascular development through manipulating the expression of cellulose and lignin biosynthesis genes. Overexpression of JcmiR172a enhanced xylem development and reduced phloem and pith development. This study helped elucidate the functions of miR172 in perennial plants, a known age-related miRNA involved in the regulation of perennial plant phase change and organ development.