Two ferritin subunits of Atlantic salmon (Salmo salar): cloning of the liver cDNAs and antibody preparation.
Two ferritin subunits of Atlantic salmon (Salmo salar): cloning of the liver cDNAs and antibody preparation.
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发表时间：
1995-06
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通讯作者：
O. Andersen;A. Dehli;H. Standal;T. A. Giskegjerde;R. Karstensen;K. Rorvik
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作者：
O. Andersen;A. Dehli;H. Standal;T. A. Giskegjerde;R. Karstensen;K. Rorvik
从大西洋鲑鱼（萨尔莫salar）肝脏中分离铁蛋白重（H）和中（M）亚基cDNA。通过筛选肝脏cDNA文库，获得了编码176个残基的铁蛋白M亚基的全长克隆。在上游非翻译区发现了进化上保守的铁响应元件（IRE）。通过对鲑鱼肝脏mRNA进行逆转录-聚合酶链反应（RT-PCR）来克隆铁蛋白H cDNA。鲑鱼铁蛋白H亚基的177个残基显示67%的序列同源性与M亚基。北方印迹分析表明，铁蛋白H mRNA在肝脏，性腺，头肾，心脏和脾脏，而M亚基mRNA被发现几乎只在性腺。针对鲑鱼铁蛋白H和M的多克隆抗体在兔中产生。
The ferritin heavy (H) and middle (M) subunit cDNAs were isolated from the Atlantic salmon (Salmo salar) liver. Full-length clones encoding the ferritin M subunit of 176 residues were obtained by screening of a liver cDNA library. The evolutionary conserved iron-responsive element (IRE) was identified in the upstream untranslated region. Ferritin H cDNA was cloned by running reverse transcription-polymerase chain reaction (RT-PCR) on salmon liver mRNA. The salmon ferritin H subunit of 177 residues showed 67% sequence identity with the M subunit. Northern blot analysis revealed ferritin H mRNA in the liver, gonads, head kidney, heart, and spleen, whereas M subunit mRNA was found almost exclusively in the gonads. Polyclonal antibodies against both salmon ferritin H and M were raised in rabbits.