Coevolution of ribosomal RNA expansion segment 7L and assembly factor Noc2p specializes the ribosome biogenesis pathway between Saccharomyces cerevisiae and Candida albicans.
Coevolution of ribosomal RNA expansion segment 7L and assembly factor Noc2p specializes the ribosome biogenesis pathway between Saccharomyces cerevisiae and Candida albicans.
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核糖体 RNA 扩展片段 7(L) 和组装因子 Noc2p 的共同进化专门研究了酿酒酵母和白色念珠菌之间的核糖体生物发生途径
DOI：
10.1093/nar/gkab218
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发表时间：
2021-05-07
影响因子：
14.9
通讯作者：
Zhao G
中科院分区：
文献类型：
--
作者：
Wang X;Yue Z;Xu F;Wang S;Hu X;Dai J;Zhao G
不同物种的核糖体共享一个进化上保守的核心，表现出部分通过添加物种特异性核糖体RNA（rRNA）而形成的柔性外壳，其功能在很大程度上未被探索。在这项研究中，我们表明，通过交换酿酒酵母25 S rRNA基因与非S。在酿酒酵母同源物中，种特异性rRNA变异引起中度至重度前rRNA加工缺陷。具体而言，白色念珠菌的rRNA取代导致严重的生长缺陷和前rRNA加工缺陷。我们观察到，这种缺陷可以主要归因于扩展片段7 L（ES 7 L）的变化，并可以通过组装因子Noc 2 p突变体（Noc 2 p-K384 R）恢复。我们发现，交换ES 7 L减弱了Noc 2 p和其他蛋白（Erb 1 p，Rrp 1 p，Rp 16 p和Rp 17 p）掺入前核糖体，并且这种影响可以通过Noc 2 p-K384 R来补偿。此外，用C.白念珠菌也能促进Noc 2 p和上述蛋白进入前核糖体，从而恢复正常生长。总之，我们的研究结果有助于阐明物种特异性rRNA变异在核糖体生物发生中所起的作用，并进一步提供证据证明rRNA扩展片段和同源组装因子的共同进化使核糖体生物发生途径专门化，从而进一步了解核糖体的功能和进化。
Ribosomes of different species share an evolutionarily conserved core, exhibiting flexible shells formed partially by the addition of species-specific ribosomal RNAs (rRNAs) with largely unexplored functions. In this study, we showed that by swapping the Saccharomyces cerevisiae 25S rRNA genes with non-S. cerevisiae homologs, species-specific rRNA variations caused moderate to severe pre-rRNA processing defects. Specifically, rRNA substitution by the Candida albicans caused severe growth defects and deficient pre-rRNA processing. We observed that such defects could be attributed primarily to variations in expansion segment 7L (ES7L) and could be restored by an assembly factor Noc2p mutant (Noc2p-K384R). We showed that swapping ES7L attenuated the incorporation of Noc2p and other proteins (Erb1p, Rrp1p, Rpl6p and Rpl7p) into pre-ribosomes, and this effect could be compensated for by Noc2p-K384R. Furthermore, replacement of Noc2p with ortholog from C. albicans could also enhance the incorporation of Noc2p and the above proteins into pre-ribosomes and consequently restore normal growth. Taken together, our findings help to elucidate the roles played by the species-specific rRNA variations in ribosomal biogenesis and further provide evidence that coevolution of rRNA expansion segments and cognate assembly factors specialized the ribosome biogenesis pathway, providing further insights into the function and evolution of ribosome.
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影响因子：
3
作者：
De Summa S;Malerba G;Pinto R;Mori A;Mijatovic V;Tommasi S
通讯作者：
Tommasi S
影响因子：
64.5
作者：
Kater L;Thoms M;Barrio-Garcia C;Cheng J;Ismail S;Ahmed YL;Bange G;Kressler D;Berninghausen O;Sinning I;Hurt E;Beckmann R
通讯作者：
Beckmann R
影响因子：
4.5
作者：
Fang, F;Phillips, S;Butler, JS
通讯作者：
Butler, JS
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
11.4
作者：
Billy, E;Wegierski, T;Filipowicz, W
通讯作者：
Filipowicz, W