The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model

The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model
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氯泵盐视紫红质的光循环。

DOI:
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发表时间:
1985
期刊:
影响因子:
11.4
通讯作者:
J. Tittor
J. Tittor
中科院分区:
生物学1区
文献类型:
--
作者:
D. Oesterhelt;P. Hegemann;J. Tittor

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测得HR578的初级光反应量子产率为0.34±0.0 2,光化学反应的量子产率为0.0 1±0.004。从稳态光照和闪光光解实验中,对HRL410的形成和衰变进行了定量描述,得到了一个孤立的、光适应的HR的光循环模型。这个模型满足关于HR的所有已知事实,包括它的光致变色行为。
The quantum yield of the primary photoreaction of HR578 was determined as øgreen = 0.34±0.02 and that of the photochemical reconversion of HRL410 into HR578 as øblue = 0.01±0.004. From steady‐state illumination and flash‐photolysis experiments, a quantitative description of HRL410 formation and decay was made leading to a model of the photocycle in isolated, light‐adapted HR. This model satisfies all known facts about HR including its photochromic behaviour.