Immunohistochemical study of dental pulp applied with 4-META/MMA-TBB adhesive resin after pulpotomy.
Immunohistochemical study of dental pulp applied with 4-META/MMA-TBB adhesive resin after pulpotomy.
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活髓切断术后应用 4-META/MMA-TBB 粘合树脂的牙髓的免疫组织化学研究。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Masaki Shimono
中科院分区：
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作者：
Masahide Nakamura;Takashi Inoue;Masaki Shimono
本研究的目的是研究应用4-Meta/MMA-TBB粘合树脂（4-Meta树脂）后，离断牙髓组织中的神经再生和增殖活性。氢氧化钙用作对照材料。在第3天，成纤维细胞样细胞在4-Meta树脂和氢氧化钙处理组中均为增殖细胞核抗原（PCNA）阳性，并且主要位于离切割面0.5 mm以内。在该区域仅观察到少数碎裂的神经丝蛋白（NFP）阳性神经纤维。在第7天和第14天，两组中PCNA阳性细胞的数量逐渐减少，并且在牙髓的切割表面附近观察到再生的NFP阳性神经纤维。21 d时，实验组距创面0.5 mm处仍可见少量PCNA阳性细胞，距创面0.15 ~ 0.2 mm处可见NFP阳性神经纤维。对照组在第21天坏死层下形成牙本质桥。PCNA阳性细胞未被发现的牙本质桥下，但NFP阳性神经纤维再生接近it. These结果表明，虽然细胞分化和神经再生延迟，伤口愈合发生后，即使应用4-Meta树脂暴露的牙髓表面的氢氧化钙应用程序相同。
The purpose of this study was to investigate nerve regeneration and proliferative activity in amputated pulp tissue after the application of 4-META/MMA-TBB adhesive resin (4-META resin). Calcium hydroxide was used as a control material. At 3 days, fibroblast-like cells were positive for proliferating cell nuclear antigen (PCNA) in both 4-META resin- and calcium hydroxide-treated groups and were located mainly within 0.5 mm from the cut surface. Only a few fragmented neurofilament protein (NFP)-positive nerve fibers were observed in this area. At 7 and 14 days, the number of PCNA-positive cells had gradually decreased and regenerated NFP-positive nerve fibers were observed close to the cut surface of the pulp in both groups. At 21 days in the experimental group, several PCNA-positive cells were still found in the area 0.5 mm from the cut surface, and NFP-positive nerve fibers were detected about 0.15-;0.2 mm from the cut surface. In contrast, a dentin bridge was produced under the necrotic layer at 21 days in the control group. PCNA-positive cells were not found underneath the dentin bridge, but NFP-positive nerve fibers had regenerated close to it. These results suggest that although cell differentiation and nerve regeneration are delayed, wound healing occurred even after the application of 4-META resin to exposed pulp surface the same as calcium hydroxide application.