Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities
Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities
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DOI：
10.1016/j.jip.2005.10.002
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
de Miranda, JR
中科院分区：
文献类型：
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作者：
Genersch, E;Yue, C;de Miranda, JR
蜜蜂（Apis mellifera）在蛹期通过狄斯瓦螨（K destructor）感染变形翅病毒（DWV）后，发育成成虫，表现出翅膀和其他形态畸形。给你。我们首次报道了熊蜂（熊蜂（Bombus terrestris），熊蜂（Bombus pascuorum））表现出类似于在临床上DWV感染的蜜蜂中所见的翅膀畸形。使用特异性RT-PCR检测DWV，然后对PCR产物进行测序，我们可以证明熊蜂确实感染了DWV。由于这种畸形的熊蜂不能存活，DWV感染可能对熊蜂种群构成严重威胁。(c)2005年爱思唯尔公司All rights reserved.
Honey bees (Apis mellifera) productively infected with Deformed wing virus (DWV) through Varroa destructor (K destructor) during pupal stages develop into adults showing wing and other morphological deformities. Here,. we report for the first time the occurrence of bumble bees (Bombus terrestris, Bombus pascuorum) exhibiting wing deformities resembling those seen in clinically DWV-infected honey bees. Using specific RT-PCR protocols for the detection of DWV followed by sequencing of the PCR products we could demonstrate that the bumble bees were indeed infected with DWV. Since such deformed bumble bees are not viable DWV infection may pose a serious threat to bumble bee populations. (c) 2005 Elsevier Inc. All rights reserved.