ACTIVATION OF DNA-BINDING ACTIVITY IN AN APPARENTLY CYTOPLASMIC PRECURSOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR
ACTIVATION OF DNA-BINDING ACTIVITY IN AN APPARENTLY CYTOPLASMIC PRECURSOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR
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DOI：
10.1016/0092-8674(88)90382-0
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发表时间：
1988-04-22
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
BAEUERLE, PA;BALTIMORE, D
在不表达. kappa的细胞中。免疫球蛋白轻链基因，κ。增强子结合蛋白NF-κ B在细胞质或细胞核部分中不明显。然而，通过变性、大小分级和复性，NF-κ B活性可以在胞质级分中显示，表明DNA结合蛋白存在，但其结合活性受到抑制。通过使用涉及解离剂脱氧胆酸钠和甲酰胺的多种方案，在来自未刺激的70 Z/3前B细胞的级分中可以发现与在来自佛波酯活化的细胞的核提取物中发现的一样多的胞质NF-κ B。我们的结论是，70 Z/3和HeLa细胞都含有明显的胞质NF-κ B，其形式没有明显的DNA结合活性，佛波醇酯都释放了结合抑制并导致易位到细胞核。
In cells that do not express .kappa. immunoglobulin light chain genes, the .kappa. enhancer-binding protein NF-.kappa.B is not evident in either cytoplasmic or nuclear fractions. By denaturation, size fractionation, and renaturation, however, NF-.kappa.B activity can be revealed in cytosolic fractions, showing that the DNA-binding protein is present but inhibited in its binding activity. By using a variety of protocols involving the dissociating agents sodium desoxycholate and formamide, as much cytosolic NF-.kappa.B can be found in the fraction from unstimulated 70Z/3 pre-B cells as is found in the nuclear extract from phorbol ester-activated cells. We conclude that both 70Z/3 and HeLa cells contain apparently cytosolic NF-.kappa.B in a form with no evident DNA-binding activity, and phorbol esters both release the inhibition of binding and cause a translocation to the nucleus.