Purification of membranes and identification of phase-specific proteins of the dimorphic pathogenic fungus Histoplasma capsulatum.
Purification of membranes and identification of phase-specific proteins of the dimorphic pathogenic fungus Histoplasma capsulatum.
复制标题
二形性病原真菌荚膜组织胞浆菌膜的纯化和相特异性蛋白的鉴定。
DOI：
10.1016/0003-9861(88)90120-8
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发表时间：
1988
影响因子：
3.9
通讯作者：
Maresca,B
中科院分区：
文献类型：
--
作者：
Kumar,BV;Maresca,B
从酵母和菌丝体阶段ofHistoplasma capsulatum质膜囊泡已被纯化和表征。纯化方法包括弹道破碎细胞的差速离心，然后通过不连续蔗糖密度梯度和平衡离心进行沉降。通过电子显微镜评估制备物的纯度。用聚丙烯酰胺凝胶分析了酵母和真菌菌丝相的膜制备物的蛋白质组成。两个形态学阶段的比较揭示了几种膜特异性蛋白表达的定量和定性差异。膜的外观的物理差异也观察到通过电子显微镜膜制剂。膜流动性的改变可能是造成两相膜泡外观差异的原因之一。
Plasma membrane vesicles from the yeast and mycelial phases ofHistoplasma capsulatumhave been purified and characterized. The method of purification involved differential centrifugation of ballistically fractured cells followed by sedimentation through discontinuous sucrose density gradient and equilibrium centrifugation. Purity of the preparation was assessed by electron microscopy. The protein composition of the membrane preparations from the yeast and mycelial phases of the fungus was analyzed by polyacrylamide gels. A comparison of the two morphologic phases revealed quantitative and qualitative differences in the expressions of several membrane-specific proteins. Physical differences in the appearance of the membranes were also observed by electron micrography of membrane preparations. Alteration in membrane fluidity may be one of the many causes for differences in the appearance of membrane vesicles in the two phases.