Glucose uptake increases relative to oxygen consumption during short-term hibernation.
Glucose uptake increases relative to oxygen consumption during short-term hibernation.
复制标题
在短期冬眠期间，葡萄糖摄取量相对于氧气消耗量增加。
DOI：
10.1007/s003950050006
复制
发表时间：
2000
影响因子：
9.5
通讯作者：
Ward,HB
中科院分区：
文献类型：
--
作者：
McFalls,EO;Baldwin,DR;Marx,D;Maxwell,K;Ward,HB
背景：虽然葡萄糖摄取增加慢性hyperperfused，存活心肌，葡萄糖摄取相对于氧气消耗的动态变化，在“短期”模型的hyperplantation.Methods：14麻醉猪仪器与液压封堵器和流量探头的近端LAD动脉。血流量减少30%，持续1小时。在基线和缺血10、30和60分钟时测定LAD区域的心肌血流量和氧摄取、游离脂肪酸、葡萄糖和乳酸。在缺血前、缺血15分钟和缺血45分钟时，在LAD区域进行ATP和磷酸肌酸（CP）的脑活检。结果：左前降支（LAD）区心肌耗氧量由2.06 ± 0.16 μmol/min/g降至1.46 ± 0.13 μmol/min/g（P < 0.05）;缺血15分钟时，透壁磷酸肌酸从基线时的7.48 ± 0.76 μmol/g湿重降至6.19 ± 0.32 μmol/g湿重（P < 0.05），但缺血45分钟时恢复正常（7.39 ± 0.56 μmol/g湿重; NS）。在10 ~ 60分钟的恒定流量减少期间，葡萄糖摄取占MVO 2的百分比从3 ± 2%增加到10 ± 2%（P < 0.05），而乳酸摄取从-9 ± 9%增加到-1 ± 2%（P < 0.05）。结论：在此短期冬眠模型中，持续性缺血时葡萄糖和乳酸摄取相对于氧消耗增加，并与生物能量学的恢复时间相一致。这一发现与糖酵解产生的ATP对持续性缺血期间能量供应的抑制是重要的这一观点一致。
Background:Although glucose uptake is increased in chronically hyperfused, viable myocardium, the dynamic changes in glucose uptake relative to oxygen consumption in “short-term” models of hibernation have not been fully explored.Methods:14 anesthetized swine were instrumented with an hydraulic occluder and flow probe on the proximal LAD artery. Blood flow was reduced ∼30% for 1 hour. Myocardial blood flow and uptake of oxygen, free fatty acids, glucose and lactate were determined in the LAD region at baseline and at 10, 30 and 60 minutes of ischemia. Transmural biopsies for ATP and creatine phosphate (CP) were obtained in the LAD region prior to and at 15 and 45 minutes of ischemia. In 5 animals, glycogen was assayed at baseline and at the end of 60 minutes of ischemia.Results:In the LAD region, myocardial oxygen consumption was reduced from 2.06 ± 0.16 μmol/min/gram to 1.46 ± 0.13 μmol/min/gram (P < 0.05). By 15 minutes of ischemia, transmural creatine phosphate fell from 7.48 ± 0.76 μmol/g-wet weight at baseline to 6.19 ± 0.32 μmol/g-wet weight (P < 0.05) but normalized by 45 minutes of ischemia (7.39 ± 0.56 μmol/g-wet weight; NS). Between 10 and 60 minutes of constant flow reduction, glucose uptake as a percentage of MVO2increased from 3 ± 2% to 10 ± 2% (P < 0.05) while lactate uptake increased from −9± 9% to −1± 2% (P < 0.05). Glycogen decreased from 27.8 ± 3.7 at baseline to 16.9 ± 1.2 μmol/g-wet weight at end-ischemia.Conclusions:In this model of short-term hibernation, glucose and lactate uptake increase relative to oxygen consumption during sustained ischemia, and temporally coinicide with the recovery of bioenergetics. The findings are consistent with the notion that glycolytically derived ATP is important for the maintainance of energy supply during sustained ischemia.