Effects of cigarette smoke on the immune response. II. Chronic exposure to cigarette smoke inhibits surface immunoglobulin-mediated responses in B cells.
Effects of cigarette smoke on the immune response. II. Chronic exposure to cigarette smoke inhibits surface immunoglobulin-mediated responses in B cells.
复制标题
香烟烟雾对免疫反应的影响。
DOI：
10.1016/0041-008x(91)90256-e
复制
发表时间：
1991
影响因子：
3.8
通讯作者：
Sopori,ML
中科院分区：
文献类型：
--
作者：
Savage,SM;Donaldson,LA;Cherian,S;Chilukuri,R;White,VA;Sopori,ML
我们以前曾报道，长期暴露在香烟烟雾中会抑制抗体形成细胞(AFC)对T依赖和T非依赖抗原的反应，并可能反映B细胞功能障碍。在这次交流中，我们扩展了这些研究，以表明长期吸烟暴露的大鼠(SM)的T细胞功能是正常的，根据它们对有丝分裂原和“名义”或同种异体抗原的反应来判断。虽然SM的B细胞对B细胞有丝分裂原脂多糖有显著的反应，但它们不能对抗μ抗体(抗-IgM)产生增殖反应，也不能对绵羊红细胞产生显著的AFC反应。根据三硝基苯化(TNP)马红细胞形成的花环数目，SM大鼠脾中TNP结合细胞(TNP-ABC)的频率与假手术对照组相当。而丹参能显著降低TNP-ABC对TNP-布鲁氏菌的增殖作用。SM B细胞反应的这些差异，即内毒素和抗μ/抗原之间的差异，可能与内毒素绕过部分膜信号转导通路的能力有关。这些结果表明，香烟烟雾影响抗原依赖的B细胞信号转导通路的早期步骤(S)。
We have previously reported that chronic exposure of rats to cigarette smoke inhibits the antibody-forming cell (AFC) response to both T-dependent and T-independent antigens and may reflect B cell dysfunction. In this communication we extend these studies to show that T cell functions are normal in chronically smoke-exposed rats (SM) as judged by their responses to mitogens and “nominal” or alloantigens. While B cells from SM respond significantly to the B cell mitogen lipopolysaccharide (LPS), they fail to proliferate in response to anti-IgM (anti-μ) or to produce significant AFC response to sheep red blood cells. On the basis of the number of rosettes formed with trinitrophenylated (TNP) horse red blood cells, the frequency of TNP-binding cells (TNP-ABC) in the spleens of SM is comparable to sham control rats. However, the proliferation of TNP-ABC to TNP-Brucella abortus is significantly decreased in SM. These differences in SM B cell responses, i.e., between LPS and anti-μ/antigen, may to be related to the ability of LPS to bypass a portion of the membrane signal transduction cascade. These results suggest that cigarette smoke affects an early step(s) in the antigen-dependent B cell signal transduction pathway.