Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems.
Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems.
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DOI：
10.3390/ijms18020408
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发表时间：
2017-02-14
影响因子：
5.6
通讯作者：
Cervelli V
中科院分区：
文献类型：
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作者：
Gentile P;Cole JP;Cole MA;Garcovich S;Bielli A;Scioli MG;Orlandi A;Insalaco C;Cervelli V
富血小板血浆（PRP）作为雄激素性脱发（AGA）的潜在治疗方法通过两种不同的临床方案进行测试，其中一组（18名参与者）接受CPunT制备系统（Biomed Device, Modena, Italy）生产的自体非活化PRP （a -PRP）半头治疗，另一组接受安慰剂半头治疗，其中所有参与者(n = 6，每组3名参与者)接受钙活化PRP （AA-PRP）治疗，该治疗由两种不同的PRP收集装置（Regen血细胞疗法或Arthrex Angel系统）之一产生。在A-PRP研究中，三种治疗间隔30天。治疗3个月后，患者的毛发扫描分析显示，目标区域的毛发数量（36±3根头发）和总头发密度（65±5根头发平方厘米）的临床改善，而在接受安慰剂治疗的头皮区域，毛发数量（1.1±1.4根头发）和密度（1.9±10.2根头发平方厘米）的改善微不足道。治疗两周后进行的显微镜评估也显示表皮厚度、Ki67+角质形成细胞和毛囊数量增加。AA-PRP治疗组接受单次注射，治疗6个月后，两种PRP收集装置的临床结果有显著差异（+90±6发cm2 vs - 73±30发cm2， Regen vs Arthrex）。两种收集装置制备的AA-PRP中生长因子浓度在钙活化后没有显著差异。
Platelet rich plasma (PRP) was tested as a potential therapy for androgenetic alopecia (AGA) through two different clinical protocols in which one population (18 participants) received half-head treatment with autologous non-activated PRP (A-PRP) produced by CPunT Preparation System (Biomed Device, Modena, Italy) and the other half-head with placebo, and a second separated population in which all participants (n = 6, 3 participants per group) received treatment with calcium-activated PRP (AA-PRP) produced from one of two different PRP collection devices (Regen Blood Cell Therapy or Arthrex Angel System). For the A-PRP study, three treatments were administered over 30-day intervals. Trichoscan analysis of patients, three months post-treatment, showed a clinical improvement in the number of hairs in the target area (36 ± 3 hairs) and in total hair density (65 ± 5 hair cm2), whereas negligible improvements in hair count (1.1 ± 1.4 hairs) and density (1.9 ± 10.2 hair cm2) were seen in the region of the scalp that received placebo. Microscopic evaluation conducted two weeks after treatment showed also an increase in epidermal thickness, Ki67+ keratinocytes, and in the number of follicles. The AA-PRP treatment groups received a singular set of injections, and six months after the treatments were administered, notable differences in clinical outcomes were obtained from the two PRP collection devices (+90 ± 6 hair cm2 versus −73 ± 30 hair cm2 hair densities, Regen versus Arthrex). Growth factor concentrations in AA-PRP prepared from the two collection devices did not differ significantly upon calcium activation.