Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells
Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells
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DOI：
10.1089/ten.2006.12.2729
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发表时间：
2006-10-01
影响因子：
--
通讯作者：
Meinel, Lorenz
中科院分区：
文献类型：
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作者：
Hofmann, Sandra;Knecht, Sven;Meinel, Lorenz
丝素蛋白支架作为组织工程软骨样组织的一种新型生物材料选择被进行了研究。将人骨髓间充质干细胞（MSCs）接种在丝素、胶原蛋白和交联胶原蛋白支架上，并在无血清软骨形成培养基中培养21天。细胞在丝素蛋白支架上比在胶原蛋白基质上增殖得更快。丝素支架上糖胺聚糖沉积的总量是胶原蛋白支架的三倍。糖胺聚糖沉积与Ⅱ型胶原蛋白和聚集蛋白聚糖基因的过度表达相一致。软骨样组织均匀地分布在整个丝素支架中，而在胶原蛋白和交联胶原蛋白系统中，组织形成局限于外缘，呈现出环形外观。圆形或角形的细胞位于丝素系统的深腔隙中，并且Ⅱ型胶原蛋白染色呈阳性。组织工程软骨构建体的聚集模量比未接种细胞的丝素支架对照组高2倍以上。这些结果表明，丝素蛋白支架是无血清培养基中自体软骨组织工程合适的生物材料基质，能够在力学性能上有所改善，同时其成分特征适合用于制造或再生软骨的持久植入物。
Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow-derived mesenchymal stem cells (MSCs) were seeded on silk, collagen, and crosslinked collagen scaffolds and cultured for 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly on the silk fibroin scaffolds than on the collagen matrices. The total content of glycosaminoglycan deposition was three times higher on silk as compared to collagen scaffolds. Glycosaminoglycan deposition coincided with overexpression of collagen type II and aggrecan genes. Cartilage-like tissue was homogeneously distributed throughout the entire silk scaffolds, while on the collagen and crosslinked collagen systems tissue formation was restricted to the outer rim, leaving a doughnut appearance. Round or angular-shaped cells resided in deep lacunae in the silk systems and stained positively for collagen type II. The aggregate modulus of the tissue-engineered cartilage constructs was more than 2-fold higher than that of the unseeded silk scaffold controls. These results suggest that silk fibroin scaffolds are suitable biomaterial substrates for autologous cartilage tissue engineering in serum-free medium and enable mechanical improvements along with compositional features suitable for durable implants to generate or regenerate cartilage.