Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
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分离负责在球蛋白染色质上赋予 DNase I 敏感结构的核蛋白亚类。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
H. Weintraub
中科院分区：
文献类型：
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作者：
S. Weisbrod;H. Weintraub
珠蛋白基因在红细胞染色质中优先对DNase I消化敏感，但在脑、成纤维细胞或输卵管染色质中不敏感。用0.35 M NaCl洗脱红细胞染色质，在单个核小体的总体结构中没有可检测到的变化;然而，在这种耗尽的染色质中，珠蛋白基因不再优先对DNA酶I敏感。用整个0.35 M NaCl级分或来自该级分的极大富集两种高迁移率族蛋白的亚类（nos. 14和17）导致珠蛋白基因的DNA酶I敏感性的成功重建。对于所有这些制备物，失活的卵清蛋白基因对DNA酶I没有表现出优先敏感性。重建的红细胞0.35 M NaCl馏分与耗尽的脑染色质导致没有优先的珠蛋白基因在脑染色质的敏感性，然而，重建的脑0.35 M NaCl馏分与耗尽的红细胞染色质导致成功重建的DNA酶I的珠蛋白基因的敏感性。因此，负责赋予DNA酶I敏感性的洗脱蛋白质可能不是组织特异性的，并且可能不识别特定的DNA序列。
The globin gene is preferentially sensitive to digestion by DNase I in erythrocyte chromatin but not in brain, fibroblast, or oviduct chromatin. Elution of the erythrocyte chromatin with 0.35 M NaCl leads to no detectable change in the gross structure of individual nucleosomes; however, in this depleted chromatin the globin gene is no longer preferentially sensitive to DNase I. Reconstitution of the depleted chromatin with either the entire 0.35 M NaCl fraction or a subclass from this fraction greatly enriched in two high mobility group proteins (nos. 14 and 17) results in the successful reconstitution of DNase I sensitivity of the globin gene. For all of these preparations, the inactive ovalbumin gene exhibited no preferential sensitivity to DNase I. Reconstitution of the erythrocyte 0.35 M NaCl fraction with depleted brain chromatin resulted in no preferential sensitivity of the globin gene in brain chromatin; however, reconstitution of the brain 0.35 M NaCl fraction with depleted erythrocyte chromatin led to successful reconstitution of DNase I sensitivity of the globin gene. Thus, the eluted proteins responsible for conferring DNase I sensitivity are probably not tissue-specific and probably do not recognize specific DNA sequences.