A ratiometric fluorescence probe for selective visual sensing of Zn2+
A ratiometric fluorescence probe for selective visual sensing of Zn2+
复制标题
DOI：
10.1021/ja054149s
复制
发表时间：
2005-11-02
影响因子：
15
通讯作者：
Sreejith, S
中科院分区：
文献类型：
--
作者：
Ajayaghosh, A;Carol, P;Sreejith, S
描述了一种基于乙烯基吡咯封端联吡啶的简单比例荧光探针，用于在水性生理 pH (6.8−7.4) 下视觉传感 Zn2+。荧光团 3a−c 在乙腈中在 537 nm 附近显示出强发射，量子产率为 0.4。在缓冲（HEPES，pH 7.2）乙腈-水混合物（9:1 v/v）中，过渡金属盐滴定至 3c 显示出 547 nm 处发射的强烈猝灭，但 Zn2+ 除外，导致 637 nm 处发射发生红移。碱金属盐和碱土金属盐不会引起 3a−c 的发射行为发生任何显着变化。在多种其他金属离子存在的情况下，Zn2+ 的结合具有高度选择性。虽然 Cu2+ 猝灭 3c 的发射，但在 Zn2+ 存在的情况下，红色发射占主导地位，表明 3c 对 Zn2+ 的偏好。作业图和 Benesi−Hildebrand 分析显示探针和金属离子之间存在 1:1 络合。具有红色发射的 Zn2+ 的选择性视觉传感非常适合生物样本的成像。
A simple ratiometric fluorescence probe based on vinylpyrrole end-capped bipyridine for the visual sensing of Zn2+under aqueous physiological pH (6.8−7.4) is described. The fluorophores3a−cshowed strong emission around 537 nm in acetonitrile with a quantum yield of 0.4. In buffered (HEPES, pH 7.2) acetonitrile−water mixture (9:1 v/v), titration of transition metal salts to3cshowed strong quenching of the emission at 547 nm except in the case of Zn2+, which resulted in a red-shifted emission at 637 nm. Alkali and alkaline earth metal salts could not induce any considerable changes to the emission behavior of3a−c. The binding of Zn2+was highly selective in the presence of a variety of other metal ions. Though Cu2+quenches the emission of3c, in the presence of Zn2+, a red emission prevails, indicating the preference of3ctoward Zn2+. Job plot and Benesi−Hildebrand analysis revealed a 1:1 complexation between the probe and the metal ion. The selective visual sensing of Zn2+with a red emission is ideally suited for the imaging of biological specimens.