Gold Nanoparticles Capped with Polyethyleneimine for Enhanced siRNA Delivery
Gold Nanoparticles Capped with Polyethyleneimine for Enhanced siRNA Delivery
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DOI：
10.1002/smll.200901513
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发表时间：
2010-01-18
期刊：
影响因子：
13.3
通讯作者：
Wang, Jun
中科院分区：
文献类型：
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作者：
Song, Wen-Jing;Du, Jin-Zhi;Wang, Jun
一种高效、安全的小干扰RNA （siRNA）递送系统是RNA干扰疗法的临床应用所必需的。以聚乙烯亚胺（PEI）为还原剂和稳定剂，成功制备了聚乙烯亚胺（PEI）包覆金纳米粒子（AuNPs），该纳米粒子通过静电相互作用以合适的重量比与siRNA结合，得到了分散良好、结构均匀、尺寸分布窄的纳米粒子。通过siRNA结合，PEI-capped AuNPs诱导MDA-MB-435s细胞中靶向绿色荧光蛋白表达更显著和增强的降低，尽管共聚焦激光扫描显微镜观察和荧光激活细胞分选分析证实了细胞中更多内化的PEI/siRNA复合物。PEI-cap的AuNPs/siRNA靶向内源性细胞周期激酶，即癌基因polo样激酶1 (PLK1)，表现出显著的基因表达下调和细胞凋亡增强，而以PEI为载体的PLK1 siRNA处理细胞时不明显。在没有表现出细胞毒性的情况下，pei覆盖的AuArPs似乎适合作为细胞内siRNA递送的潜在载体。
An efficient and safe delivery system for small interfering RNA (siRNA) is required for clinical application of RNA interfering therapeutics. Polyethyleneimine (PEI)-capped gold nanoparticles (AuNPs) are successfully manufactured using PEI as the reductant and stabilizer, Which bind siRNA at an appropriate weight ratio by electrostatic interaction and result in well-dispersed nanoparticles with uniform structure and narrow size distribution. With siRNA binding, PEI-capped AuNPs induce more significant and enhanced reduction in targeted green fluorescent protein expression in MDA-MB-435s cells, though more internalized PEI/siRNA complexes in cells are evidenced by confocal laser scanning microscopy observation and fluorescence-activated cell sorting analyses. PEI-capped AuNPs/siRNA targeting endogenous cell-cycle kinase, an oncogene polo-like kinase 1 (PLK1), display significant gene expression knockdown and induce enhanced cell apoptosis, whereas it is not obvious when the cells are treated with PLK1 siRNA using PEI as the carrier. Without exhibiting cellular toxicity, PEI-capped AuArPs appear to be suitable as a potential carrier for intracellular siRNA delivery.