Characterization and optimization of an entropic trap for DNA separation
Characterization and optimization of an entropic trap for DNA separation
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DOI：
10.1021/ac0107002
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发表时间：
2002-01-15
影响因子：
7.4
通讯作者：
Craighead, HG
中科院分区：
文献类型：
--
作者：
Han, JY;Craighead, HG
最近，一种微制造的熵阱阵列被证明是有用的，在没有筛分基质的微芯片平台上，通过直流电泳有效地分离大的（5-200 kbp）DNA分子（在类似于30分钟内）。本文报道了该技术的进一步发展，重点是优化分离选择性和分辨率。为了预测改变各种结构参数的影响，对DNA分子与规则间隔的熵垒的相互作用进行了建模。的选择性（差分迁移率）被证明是依赖于深和浅沟道区的深度，施加的电场，和熵势垒的数量。将实验数据与模型预测值进行了比较。从模型中可以预期，在低场（严重捕获）极限下，分离分辨率应仅取决于熵阱的数量。然而，实际上，分辨率确实取决于所施加的场，因为DNA的弛豫在高场下无法实现。探讨了熵阱阵列装置分离兆碱基对DNA的必要性和可行性。
Recently, a microfabricated entropic trap array was demonstrated to be useful in separating large (5-200 kbp) DNA molecules efficiently (within similar to30 min), by dc electrophoresis, on a microchip platform without a sieving matrix. This paper reports further development of the technique, with emphasis on optimizing separation selectivity and resolution. The interaction of DNA molecules with regularly spaced entropic barriers was modeled in order to predict the effect of changing various structural parameters. The selectivity (differential mobility) was shown to be dependent on the depth of deep and shallow channel regions, applied electric field, and number of entropic barriers. Experimental data were compared with the prediction of the model. It was expected from the model that, in the low-field (severe trapping) limit, separation resolution should depend only on the number of entropic traps. However, in reality, resolution did depend on the applied field because the relaxation of DNA is not achieved at high fields. The requirement and feasibility of megabase pair DNA separation with the entropic trap array device was discussed.