The spatial landscape of T cells in the microenvironment of stage III lung adenocarcinoma
The spatial landscape of T cells in the microenvironment of stage III lung adenocarcinoma
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DOI：
10.1002/path.6254
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发表时间：
2024-02-16
影响因子：
7.3
通讯作者：
Wei，Feng
中科院分区：
文献类型：
--
作者：
Zeng，Ziqing;Du，Weijiao;Wei，Feng
本研究旨在通过分析T细胞空间格局为患者的预后分层提供更多信息。它涉及使用多重免疫荧光分析80例III期肺腺癌（LUAD）患者的染色组织切片，并探索肿瘤中心（CT）和浸润边缘（IM）的T细胞空间景观及其与预后的关系。多因素回归分析显示，CT CD 4+常规T细胞（Tconv）与诱导性Treg（iTreg）、自然调节性T细胞（nTreg）与Tconv、终末CD 8 +T细胞（tCD 8）与辅助性T细胞（Th）、IM Treg与tCD 8的相对聚集度以及CT nTreg与iTreg、IM nTreg与nTreg的相对离散度是DFS的独立危险因素。最后，我们构建了一个名为GT评分的空间免疫学评分，它比基于CD 3/CD 8细胞密度的IMMUNOSCORE®具有更强的预后相关性。肿瘤微环境中T细胞的空间分布和拟定的GT评分比T细胞密度更能反映III期LUAD患者的预后。对T细胞空间格局的探索可能提示潜在的细胞-细胞相互作用和治疗靶点，并更好地指导临床决策。© 2024大不列颠和爱尔兰病理学会。
This study aimed to provide more information for prognostic stratification for patients through an analysis of the T‐cell spatial landscape. It involved analyzing stained tissue sections of 80 patients with stage III lung adenocarcinoma (LUAD) using multiplex immunofluorescence and exploring the spatial landscape of T cells and their relationship with prognosis in the center of the tumor (CT) and invasive margin (IM). In this study, multivariate regression suggested that the relative clustering of CT CD4+conventional T cell (Tconv) to inducible Treg (iTreg), natural regulatory T cell (nTreg) to Tconv, terminal CD8+T cell (tCD8) to helper T cell (Th), and IM Treg to tCD8 and the relative dispersion of CT nTreg to iTreg, IM nTreg to nTreg were independent risk factors for DFS. Finally, we constructed a spatial immunological score named the GTscore, which had stronger prognostic correlation than IMMUNOSCORE® based on CD3/CD8 cell densities. The spatial layout of T cells in the tumor microenvironment and the proposed GTscore can reflect the prognosis of patients with stage III LUAD more effectively than T‐cell density. The exploration of the T‐cell spatial landscape may suggest potential cell–cell interactions and therapeutic targets and better guide clinical decision‐making. © 2024 The Pathological Society of Great Britain and Ireland.