Enhanced nerve regeneration through a bilayered chitosan tube: the effect of introduction of glycine spacer into the CYIGSR sequence.
Enhanced nerve regeneration through a bilayered chitosan tube: the effect of introduction of glycine spacer into the CYIGSR sequence.
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DOI：
10.1002/jbm.a.31522
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
Wei Wang;S. Itoh;A. Matsuda;T. Aizawa;M. Demura;S. Ichinose;K. Shinomiya;J. Tanaka
中科院分区：
文献类型：
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作者：
Wei Wang;S. Itoh;A. Matsuda;T. Aizawa;M. Demura;S. Ichinose;K. Shinomiya;J. Tanaka
我们开发了一种新型的双层壳聚糖管，其包括壳聚糖膜的外层和壳聚糖非织造纳米/微米纤维网的内层。该管是用静电纺丝方法制造的。我们表征了这种材料的微观结构和力学性能。我们将甘氨酸间隔区引入CYIGSR序列中，CYIGSR序列是层粘连蛋白-1的结构域，其增强雪旺细胞的迁移和附着以及神经生长，从而产生氨基酸序列CGGYIGSR和CGGGGGYIGSR。将这些肽共价结合到壳聚糖管的纳米/微米纤维网表面，以检查肽移动性对神经再生的影响。将这些双层壳聚糖管构建成支架移植到损伤的坐骨神经上。进行同种移植作为对照。这些支架在植入后5周和10周被移除用于组织学分析。神经再生成壳聚糖管，其上的CGGGGGGYIGSR肽固定，表现出类似的同系移植物的功效，并代表一个有前途的候选人，促进周围神经修复。
We have developed a novel bilayered chitosan tube that comprises an outer layer of chitosan film and an inner layer of chitosan nonwoven nano/microfiber mesh. The tube is fabricated with an electrospinning method. We characterized the microstructure and mechanical properties of this material. We introduced glycine spacers into the CYIGSR sequence, domain of laminin-1 that enhances Schwann cells migration and attachment, as well as neural outgrowth, resulting in the amino acid sequences CGGYIGSR and CGGGGGGYIGSR. These peptides were covalently bound to the nano/microfiber mesh surface of the chitosan tube to examine the effects of peptide mobility on nerve regeneration. Scaffolds constructed from these bilayered chitosan tubes were grafted to bridge injured sciatic nerve. Isografting was performed as a control. These scaffolds were removed 5 and 10 weeks after implantation for histological analysis. Nerve regeneration into chitosan tubes, on which the CGGGGGGYIGSR peptide was immobilized, exhibited efficacy similar to that of the isograft and represent a promising candidate for promoting peripheral nerve repair.