α-synuclein immunoreactivity in neuronal nuclear inclusions and neurites in multiple system atrophy
α-synuclein immunoreactivity in neuronal nuclear inclusions and neurites in multiple system atrophy
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DOI：
10.1016/j.neulet.2003.09.075
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发表时间：
2004-01-09
影响因子：
2.5
通讯作者：
Dickson, DW
中科院分区：
文献类型：
--
作者：
Lin, WL;DeLucia, MW;Dickson, DW
我们使用α -突触核蛋白抗体检测了多系统萎缩脑脑桥底的神经元和少突胶质核包涵体和神经突。免疫组化显示部分神经元和少突胶质细胞核内α -葡糖蛋白阳性包涵体。免疫电镜显示，标记的内含物由直径为10 ~ 20nm的成束紧密排列的直细丝组成。这些细丝在形态和免疫反应性上与神经元和少突胶质细胞胞体中的细丝相似，如果不是完全相同的话。此外，在树突或无髓鞘轴突中发现了类似的免疫阳性细丝，而在有髓鞘轴突中则没有。这些包涵体在转录和轴突功能障碍方面的功能意义尚不清楚。2003爱思唯尔爱尔兰有限公司版权所有。
We examined neuronal and oligodendroglial nuclear inclusions and neurites in the pontine base of multiple system atrophy brains using an antibody to alpha-synuclein. Immunohistochemistry showed alpha-syrtuclein positive inclusions in the nuclei of some neurons and oligodendroglia. Immunoelectron microscopy showed that the labeled inclusions were composed of bundles of tightly packed straight filaments with a diameter of 10-20 nm. The filaments were similar, if not identical, in morphology and immunoreactivity, to those found in the soma of neurons and oligodendrocytes with glial cytoplasmic inclusions. In addition, similar immuno-positive filaments were found in dendrites or unmyelinated axons, but not in myelinated axons. The functional significance of these inclusions in terms of transcriptional and axonal dysfunction is unknown. (C) 2003 Elsevier Ireland Ltd. All rights reserved.