Similarities and differences between porcine mandibular and limb bone marrow mesenchymal stem cells.
Similarities and differences between porcine mandibular and limb bone marrow mesenchymal stem cells.
复制标题
DOI：
10.1016/j.archoralbio.2017.01.012
复制
发表时间：
2017-05
影响因子：
3
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Lloyd B;Tee BC;Headley C;Emam H;Mallery S;Sun Z
研究表明，使用骨髓间充质干细胞（BMSCs）的颅面骨再生的承诺，但鲜为人知的是，从四肢和颅面骨的BMSCs的差异。本研究比较了猪下颌骨和胫骨BMSCs的体外增殖、成骨分化特性和基因表达。从3-4月龄的猪（n=4）的胫骨和下颌骨抽吸骨髓，随后进行BMSC分离、培养扩增和通过流式细胞术表征。使用群体倍增时间评估增殖率。成骨分化以碱性磷酸酶活性评价。采用Affyellow猪基因芯片比较胫骨和下颌骨BMSCs的基因表达，然后进行某些基因的实时RT-PCR评价。我们的研究结果表明，从两个位置的BMSCs表达MSC标记，但不造血标记。下颌骨BMSCs的增殖和成骨分化能力明显强于胫骨BMSCs。微阵列分析确定了404个高丰度基因，其中334个基因在两个位置之间匹配并注释到相同的功能组，包括骨生成和血管生成，而70个基因不匹配并注释到不同的功能组。此外，48个基因在两个位置之间差异表达至少1.5倍，包括颅神经嵴相关基因BMP-4在下颌骨BMSCs中的较高表达，这一点通过实时RT-PCR得到证实。总之，这些数据表明，尽管下颌骨和胫骨BMSCs之间的基因表达有很强的相似性，下颌骨BMSCs表达的一些基因与胫骨BMSCs不同，并具有表型特征，可能使它们有利于颅面骨再生。
Research has shown promise of using bone marrow mesenchymal stem cells (BMSCs) for craniofacial bone regeneration; yet little is known about the differences of BMSCs from limb and craniofacial bones. This study compared pig mandibular and tibia BMSCs for their in vitro proliferation, osteogenic differentiation properties and gene expression. Bone marrow was aspirated from the tibia and mandible of 3–4 month-old pigs (n=4), followed by BMSC isolation, culture-expansion and characterization by flow cytometry. Proliferation rates were assessed using population doubling times. Osteogenic differentiation was evaluated by alkaline phosphatase activity. Affymetrix porcine microarray was used to compare gene expressions of tibial and mandibular BMSCs, followed by real-time RT-PCR evaluation of certain genes. Our results showed that BMSCs from both locations expressed MSC markers but not hematopoietic markers. The proliferation and osteogenic differentiation potential of mandibular BMSCs were significantly stronger than those of tibial BMSCs. Microarray analysis identified 404 highly abundant genes, out of which 334 genes were matched between the two locations and annotated into the same functional groups including osteogenesis and angiogenesis, while 70 genes were mismatched and annotated into different functional groups. In addition, 48 genes were differentially expressed by at least 1.5-fold difference between the two locations, including higher expression of cranial neural crest-related gene BMP-4 in mandibular BMSCs, which was confirmed by real-time RT-PCR. Altogether, these data indicate that despite strong similarities in gene expression between mandibular and tibial BMSCs, mandibular BMSCs express some genes differently than tibial BMSCs and have a phenotypic profile that may make them advantageous for craniofacial bone regeneration.