REGULATION OF MELANIN SYNTHESIS IN MAMMALIAN CELLS AS STUDIED BY SOMATIC HYBRIDIZATION .I. EVIDENCE FOR NEGATIVE CONTROL
REGULATION OF MELANIN SYNTHESIS IN MAMMALIAN CELLS AS STUDIED BY SOMATIC HYBRIDIZATION .I. EVIDENCE FOR NEGATIVE CONTROL
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DOI：
10.1002/jcp.1040720206
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发表时间：
1968-01-01
影响因子：
5.6
通讯作者：
YAMAMOTO, K
中科院分区：
文献类型：
--
作者：
DAVIDSON, R;EPHRUSSI, B;YAMAMOTO, K
对叙利亚仓鼠染色细胞与小鼠非染色细胞进行了分离和体外繁殖。这些杂交种没有色素，缺乏多巴氧化酶（和酪氨酸酶）活性，这与叙利亚仓鼠细胞的色素沉着有关。相比之下，在杂交细胞中观察到其他三种酶（LDH、MDH和胸苷激酶）的存在，这些酶也由仓鼠基因组指定，但与色素合成无关。这表明多巴氧化酶在这些细胞中的抑制是对与色素细胞分化状态相关的酶的特异性影响。因此，在这种情况下，分化的遗传控制涉及一种负作用的扩散调节物质。
Somatic hybrids between pigmented Syrian hamster cells and unpigmented mouse cells were isolated and propagatedin vitro. These hybrids are unpigmented and lack dopa oxidase (and tyrosinase) activity, which is correlated with the pigmentation of the Syrian hamster cells. In contrast, the presence of three other enzymes (LDH, MDH, and thymidine kinase) also specified by the hamster genome but unrelated to pigment synthesis is observed in the hybrid cells. This suggests that the repression of dopa oxidase in these cells is a specific effect on the enzyme associated with the differentiated state of pigment cells. It is concluded that the genetic control of differentiation in this case involves a diffusible regulator substance which functions negatively.