Screening of novel matrix metalloproteinases (MMPs) in human fetal membranes
Screening of novel matrix metalloproteinases (MMPs) in human fetal membranes
复制标题
DOI：
10.1023/a:1020362519981
复制
发表时间：
2002-10-01
影响因子：
3.1
通讯作者：
Menon, R
中科院分区：
文献类型：
--
作者：
Fortunato, SJ;Menon, R
目的：人类胎膜中基质金属蛋白酶（MMP）的内源性激活可能导致胎膜弱化，导致胎膜早期破裂，并参与分娩的开始。我们的实验室和其他几个实验室已经研究了其中一些MMPs的来源和作用。本研究的目的是记录到目前为止克隆和测序的大多数MMPs在羊膜早破（pPROM）、足月非产程和足月产程期间的表达模式。材料和方法：收集胎膜早破、剖腹产后足月未产程和足月阴道分娩的妇女的胎盘。从胎盘上分离膜，选择远离破裂部位的切片。羊膜绒毛膜与胎盘分离。利用特异性引物RT-PCR检测MMP15 （MT2-MMP）、MMP16 （MT3-MMP）、MMP17 （MT4-MMP）、MMP18、MMP20、MMP23、MMP24 （MT5-MMP）、MMP25 （MT6-MMP）和MMP 26的表达模式。结果：本研究中筛选的一些新型MMPs在人胎膜中的表达模式存在差异。大部分MMPs的mRNA均通过羊膜表达。MMP16[膜型金属蛋白酶3]、MMP20[漆膜溶素]、MMP26[基质溶素]未表达。结论：羊膜在pPROM时、非产程和产程时表达了多个MMP基因。我们之前已经报道了其他MMPs及其抑制剂的表达模式及其在PROM中的潜在作用。这些发现支持了我们的假设，即羊膜有一个功能齐全的MMP系统。
Objective: Endogenous activation of matrix metalloproteinase (MMP) in human fetal membranes is hypothesized to contribute to membrane weakening leading to early rupture and is also involved in the initiation of labor. Our laboratory and several others have studied the source and action of some of these MMPs. The objective of this study is to document the expression pattern of most of the MMPs cloned and sequenced so far in amniochorion during preterm premature rupture of membranes (pPROM), at term not in labor and during term labor.Materials and Methods: Placentas were collected from women with PROM, term not in labor after C-sections and from women after term vaginal delivery. Membranes were separated from the placenta and a section away from the rupture site was selected. Amniochorion were separated from the placenta. RT-PCR was performed to study the expression pattern of MMP15 (MT2-MMP), MMP16 (MT3-MMP), MMP17 (MT4-MMP), MMP18, MMP20, MMP23, MMP24 (MT5-MMP), MMP25 (MT6-MMP), and MMP 26 using specific primers.Results: A differential pattern of expression was noted for some of the novel MMPs screened in this study in human fetal membranes. mRNA for most of the MMPs were expressed by amniochorion. MMP16 [membrane type metalloproteinase 3], MMP20 [enamelysin], and MMP26 [matrilysin] were not expressed.Conclusion: Amniochorion expresses several of the MMP genes at the time of pPROM, term not in labor and during active labor. We have previously reported the expression pattern of other MMPs and their inhibitors and their potential role in PROM. These findings support our hypothesis that amniochorion has a fully functional MMP system.