In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis
In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis
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DOI：
10.1152/jn.00620.2011
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发表时间：
2012-03-01
影响因子：
2.5
通讯作者：
Wightman, R. Mark
中科院分区：
文献类型：
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作者：
Herr, Natalie R.;Park, Jinwoo;Wightman, R. Mark
Herr NR，Park J，McElligott ZA，贝儿AM，Carelli RM，Wightman RM.终纹床核亚区电诱发细胞外去甲肾上腺素的在体伏安法监测。J Neurophysiol 107：1731-1737，2012.首次发表于2011年12月21日; doi：10.1152/jn.00620.2011.-去甲肾上腺素（NE）是一种容易氧化的神经递质，在整个大脑中发现。大量证据表明，它在与恐惧和焦虑反应相关的神经回路中起着重要作用。在终纹床核（BNST）的某些亚区中，发现大量NE。在这项工作中，我们探讨了电诱发NE释放的差异和去甲肾上腺素转运体（NET）和α（2）-肾上腺素能自身受体（α（2）-AR）的调节在两个区域的BNST麻醉大鼠。NE监测背内侧BNST（dmBNST）和腹侧BNST（vBNST）的快速扫描循环伏安法在碳纤维微电极。通过全身应用（腹膜内注射）或局部应用（离子电渗疗法）引入药理学试剂。将离子电渗筒连接到碳纤维微电极，以允许同时检测诱发的NE释放和定量离子电渗递送。地昔帕明（Desipramine，DSI）是一种NET的抑制剂，它能增加NE的诱发释放，并能减缓NE在两个区域的清除，与释放方式无关。然而，在vBNST中的抑制作用比在dmBNST中更强。同样，α（2）-AR自身受体抑制剂idazoxan（IDA）增强NE释放在这两个区域，但在更大程度上在vBNST通过两种模式的交付。由于局部应用离子导入和全身应用IDA对NE释放的影响相似，我们的研究结果表明，末端自身受体在抑制随后的释放中起着主导作用。
Herr NR, Park J, McElligott ZA, Belle AM, Carelli RM, Wightman RM. In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis. J Neurophysiol 107: 1731-1737, 2012. First published December 21, 2011; doi:10.1152/jn.00620.2011.-Norepinephrine (NE) is an easily oxidized neurotransmitter that is found throughout the brain. Considerable evidence suggests that it plays an important role in neurocircuitry related to fear and anxiety responses. In certain subregions of the bed nucleus of the stria terminalis (BNST), NE is found in large amounts. In this work we probed differences in electrically evoked release of NE and its regulation by the norepinephrine transporter (NET) and the alpha(2)-adrenergic autoreceptor (alpha(2)-AR) in two regions of the BNST of anesthetized rats. NE was monitored in the dorsomedial BNST (dmBNST) and ventral BNST (vBNST) by fast-scan cyclic voltammetry at carbon fiber microelectrodes. Pharmacological agents were introduced either by systemic application (intraperitoneal injection) or by local application (iontophoresis). The iontophoresis barrels were attached to a carbon fiber microelectrode to allow simultaneous detection of evoked NE release and quantitation of iontophoretic delivery. Desipramine (DMI), an inhibitor of NET, increased evoked release and slowed clearance of released NE in both regions independent of the mode of delivery. However, the effects of DMI were more robust in the vBNST than in the dmBNST. Similarly, the alpha(2)-AR autoreceptor inhibitor idazoxan (IDA) enhanced NE release in both regions but to a greater extent in the vBNST by both modes of delivery. Since both local application by iontophoresis and systemic application of IDA had similar effects on NE release, our results indicate that terminal autoreceptors play a predominant role in the inhibition of subsequent release.