Fuling Granule, a Traditional Chinese Medicine Compound, Suppresses Cell Proliferation and TGFβ-Induced EMT in Ovarian Cancer.
Fuling Granule, a Traditional Chinese Medicine Compound, Suppresses Cell Proliferation and TGFβ-Induced EMT in Ovarian Cancer.
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中药复方茯苓颗粒抑制卵巢癌细胞增殖和 TGFβ 诱导的 EMT
DOI：
10.1371/journal.pone.0168892
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Shen M
中科院分区：
文献类型：
--
作者：
Tao F;Ruan S;Liu W;Wang L;Xiong Y;Shen M
复方茯苓颗粒是我国治疗卵巢癌的传统中药，已有二十多年的历史。然而，其抗癌作用的潜在分子机制仍不清楚。在这项研究中，芯片数据分析，以寻找差异表达的基因在CFG处理卵巢癌细胞。鉴定了几个细胞周期和上皮-间质转化（EMT）相关基因。微阵列分析还显示，CFG可能调节卵巢癌中的EMT。我们还发现，在功能上，CFG通过细胞周期阻滞、凋亡和衰老显著抑制卵巢癌细胞的增殖，可能与AKT/GSK-3β通路有关。此外，CFG显著抑制TGFβ诱导的卵巢癌的侵袭和迁移能力。总之，我们的结果表明，CFG抑制卵巢癌细胞增殖以及TGFβ1诱导的EMT在体外。最后，我们发现，在体内，CFG抑制肿瘤生长和远处转移。总的来说，这些发现为设计新的癌症临床治疗方法提供了有用的线索。
The compound fuling granule (CFG) is a traditional Chinese drug which has been used to treat ovarian cancer in China for over twenty years. Nevertheless, the underlying molecular mechanism of its anti-cancer effect remains unclear. In this study, microarray data analysis was performed to search differentially expressed genes in CFG-treated ovarian cancer cells. Several cell cycle and epithelial-mesenchymal transition (EMT) related genes were identified. The microarray analyses also revealed that CFG potentially regulates EMT in ovarian cancer. We also found that, functionally, CFG significantly suppresses ovarian cancer cell proliferation by cell cycle arrest, apoptosis and senescence and the AKT/GSK-3β pathway is possibly involved. Additionally, the invasion and migration ability of ovarian cancer induced by TGFβ is significantly suppressed by CFG. In conclusion, our results demonstrated that CFG suppresses ovarian cancer cell proliferation as well as TGFβ1-induced EMT in vitro. Finally, we discovered that CFG suppresses tumor growth and distant metastasis in vivo. Overall, these findings provide helpful clues to design novel clinical treatments against cancer.
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DOI：
10.1155/2014/146383
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Kong S;Zhang YH;Liu CF;Tsui I;Guo Y;Ai BB;Han FJ
通讯作者：
Han FJ
DOI：
10.1155/2015/316872
发表时间：
2015
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Park B;You S;Jung J;Lee JA;Yun KJ;Lee MS
通讯作者：
Lee MS
影响因子：
3.7
作者：
Li SG;Chen HY;Ou-Yang CS;Wang XX;Yang ZJ;Tong Y;Cho WC
通讯作者：
Cho WC
影响因子：
3.4
作者：
Lee, Sarah E.;Bairstow, Shawn F.;Hoffman, Robert M.
通讯作者：
Hoffman, Robert M.
影响因子：
0.9
作者：
Shirakawa, H.;Ishida, H.;Tanabe, K.
通讯作者：
Tanabe, K.