Survey on the contamination of Toxoplasma gondii oocysts in the soil of public parks of Wuhan, China
Survey on the contamination of Toxoplasma gondii oocysts in the soil of public parks of Wuhan, China
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DOI：
10.1016/j.vetpar.2011.08.025
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发表时间：
2012-03-23
影响因子：
2.6
通讯作者：
Zhao, J. L.
中科院分区：
文献类型：
--
作者：
Du, F.;Feng, H. L.;Zhao, J. L.
弓形虫是温血动物和人的重要病原体。土壤日益被认为是弓形虫传播的重要来源。为了解公园土壤中弓形虫的污染状况，于2009年9月至2010年8月在武汉、湖北、中国等6个公园的不同地点采集了252份土壤样品，采用聚合酶链式反应和环介等温扩增技术(LAMP)进行检测。对土壤中速殖子的检测下限分别为50、5、5。两种基因均检出弓形虫阳性41例，检出LAMP产物58例(X~2=3.6328，P=0.0567)。所有公园均被污染，各公园间差异无统计学意义(PCR值：CH2=0.0072)。P=0.9325；灯：CHI(2)=0.6101，P=0.4347)。但四季间污染差异显著(PCRCHI(2)=11.6066，P=0.0007；LAMP：CHI(2)=12.4636，P=0.0004)，从春季到冬季，感染率均呈逐渐下降的趋势。这是对中国公园土壤中弓形虫卵囊污染的首次调查。结果表明，受弓形虫卵囊污染的公园土壤可能在弓形虫病的流行中起作用，应考虑采取有效的预防措施。此外，本研究所用的常规PCR和LAMP方法也适用于土壤样品中弓形虫卵囊的检测。(C)2011爱思唯尔B.V.保留所有权利。
Toxoplasma gondii of warm-blooded animals and humans is an important pathogenic agent throughout the world. Soil is increasingly recognized as an important source in the transmission of Toxoplasma. To attain the contamination status of T. gondii in the soil of public parks, a total of 252 soil samples were collected from September 2009 to August 2010 at different sites located in 6 public parks of Wuhan, Hubei, China and detected by PCR and loop-mediated isothermal amplification (LAMP). The detection limit of PCR/B1, PCR/529 and LAMP was determined to be 50, 5, and 5 tachyzoites in soil, respectively. Forty-one samples were found positive for Toxoplasma DNA by PCR on both genes, whereas LAMP products were generated in 58 samples (chi(2) = 3.6328, P = 0.0567). All parks were found contaminated and no significant difference was found among the parks (PCR: chi(2) = 0.0072. P = 0.9325; LAMP: chi(2) = 0.6101, P = 0.4347). However, contamination was found with significantly different among the four seasons (PCR: chi(2) = 11.6066, P = 0.0007; LAMP: chi(2) = 12.4636, P = 0.0004), with a gradual decrease in the prevalence from spring to winter on both analyses. This is the first investigation on soil contamination of public parks in China by T. gondii oocysts. The results indicate that the soil of public parks contaminated with T. gondii oocysts may play a role in the epidemiology of toxoplasmosis and effective preventive measures should be considered. Moreover, the conventional PCR and LAMP used in the present study are applicable to detect T. gondii oocysts in soil samples. (C) 2011 Elsevier B.V. All rights reserved.