Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids
Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids
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DOI：
10.1016/j.tiv.2013.10.006
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发表时间：
2014-03-01
影响因子：
3.2
通讯作者：
Talbot, P.
中科院分区：
文献类型：
--
作者：
Behar, R. Z.;Davis, B.;Talbot, P.
在之前的一项关于电子烟(EC)灌装液的研究中，肉桂锡兰是36种测试产品中细胞毒性最强的。本研究的目的是确定高细胞毒性是否是肉桂味EC再灌装液的一般特征，并确定肉桂锡兰中的有毒物质(S)。用MIT试验筛选的八种肉桂口味的再灌注液，其细胞毒性各不相同，其中大多数是细胞毒性。人类胚胎干细胞通常比人类成年肺成纤维细胞更敏感。大多数产品是高度挥发性的，产生的蒸汽损害了相邻油井中细胞的存活。采用气相色谱-质谱法和高压液相色谱联用技术，对肉桂风味再灌注液中的肉桂醛(CAD)、2-甲氧基肉桂醛(2MOCA)、二丙醇和香草醛进行了检测。当用MU法检测每种化学物质的正品时，只有CAD和2MOCA具有高度的细胞毒性。用高效液相色谱法测定再灌注液中每种化学物质的含量，细胞毒性与CAD/产品的量相关。相同产品的复制瓶相似，但2MOCA浓度不同。这些数据表明，补充液中的肉桂调味料与细胞毒性有关，这可能会对EC用户产生不利影响。(C)2013爱思唯尔有限公司。保留所有权利。
In a prior study on electronic cigarette (EC) refill fluids, Cinnamon Ceylon was the most cytotoxic of 36 products tested. The purpose of the current study was to determine if high cytotoxicity is a general feature of cinnamon-flavored EC refill fluids and to identify the toxicant(s) in Cinnamon Ceylon. Eight cinnamon-flavored refill fluids, which were screened using the MIT assay, varied in their cytotoxicity with most being cytotoxic. Human embryonic stem cells were generally more sensitive than human adult pulmonary fibroblasts. Most products were highly volatile and produced vapors that impaired survival of cells in adjacent wells. Cinnamaldehyde (CAD), 2-methoxycinnamaldehyde (2MOCA), dipropylene glycol, and vanillin were identified in the cinnamon-flavored refill fluids using gas chromatography-mass spectrometry and high-pressure liquid chromatography (HPLC). When authentic standards of each chemical were tested using the MU assay, only CAD and 2MOCA were highly cytotoxic. The amount of each chemical in the refill fluids was quantified using HPLC, and cytotoxicity correlated with the amount of CAD/product. Duplicate bottles of the same product were similar, but varied in their concentrations of 2MOCA. These data show that the cinnamon flavorings in refill fluids are linked to cytotoxicity, which could adversely affect EC users. (C) 2013 Elsevier Ltd. All rights reserved.