THE PRODUCT OF RAB2, A SMALL GTP BINDING-PROTEIN, INCREASES NEURONAL ADHESION, AND NEURITE GROWTH-INVITRO
THE PRODUCT OF RAB2, A SMALL GTP BINDING-PROTEIN, INCREASES NEURONAL ADHESION, AND NEURITE GROWTH-INVITRO
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DOI：
10.1016/0896-6273(90)90206-u
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发表时间：
1990-05-01
期刊：
影响因子：
16.2
通讯作者：
PROCHIANTZ, A
中科院分区：
文献类型：
--
作者：
AYALA, J;TOUCHOT, N;PROCHIANTZ, A
rab基因编码小CTP结合蛋白，其与p2lras共享结合和水解GTP的能力。它们与YfT7和SfC4的产物存在显著的序列同源性，YfT7和SfC4是参与酵母分泌调节的两种小CTP结合蛋白。几个tab基因在发育和成年小鼠大脑中表达。为了直接检测这些基因在神经元分化中的可能作用，我们用大肠杆菌中产生的纯化rab蛋白。大肠杆菌介导的神经元从E15大鼠中脑分离。最显著的效果是用rab2蛋白（rab2p）获得的。与未处理的细胞相比，负载rab2p的神经元与培养基质的粘附性增强。这种现象在接种后3小时可见，随后在24小时内神经突生长急剧增加。用四种其它RAB基因的产物加载相同的神经元群体，或者降低神经元粘附和神经突生长，或者没有效果。这些实验表明rab2p的表达在神经元分化中起重要作用。
The rab genes code for small CTP binding proteins that share with p2lras the ability to bind and hydrolyze GTP. They present significant sequence homologies with the products of YfT7 and SfC4, two small CTP binding proteins involved in the regulation of secretion in the yeast. Several tab genes are expressed in the developing and adult mouse brain. To test directly the possible involvement of these genes in neuronal differentiation, purified rab proteins produced in E. coli were introduced into neurons dissociated from El5 rat midbrain. The most striking effects were obtained with rab2 protein(rab2p). Compared with untreated cells, neurons loaded with rab2p presented an enhanced adhesion to the culture substratum. This phenomenon was visible 3 hr after seeding and was followed within 24 hr by a dramatic increase in neurite growth. loading the same population of neurons with the products of four other rab genes either decreased neuronal adhesion and neurite growth or had no effect. These experiments suggest that the expression of rab2p plays an important role in neuronal differentiation.