STABILIZATION OF THE COLCHICINE-BINDING ACTIVITY OF TUBULIN BY ORGANIC-ACIDS
STABILIZATION OF THE COLCHICINE-BINDING ACTIVITY OF TUBULIN BY ORGANIC-ACIDS
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DOI：
10.1016/0304-4165(81)90231-2
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
LIN, CM
中科院分区：
文献类型：
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作者：
HAMEL, E;LIN, CM
许多羧酸和有机磷酸盐在稳定牛脑微管蛋白的秋水仙素结合活性方面是非常有效的。最活跃的是谷氨酸、戊二酸、δ-谷氨酸。氨基戊酸、葡萄糖1-磷酸、G-6-P、果糖1，6-（二）磷酸、磷酸肌酸和6-磷酸葡萄糖酸。最大效应发生在高浓度下。还检查了试剂的组合，发现用于稳定微管蛋白的最有效的混合物是1.0M谷氨酸盐、100 mM葡萄糖1-磷酸盐、1 mM GTP和0.5mg/ml白蛋白的组合。在37 ℃下48小时内没有发生活性损失。当微管蛋白以100 μ g/ml的浓度存在时，
A number of carboxylic acids and organic phosphates were highly effective in stabilizing the colchicine-binding activity of calf brain tubulin. The most active were glutamate, glutarate, .delta.-aminovalerate, glucose 1-phosphate, G-6-P, fructose 1,6-(bis)phosphate, creatine phosphate and 6-phosphogluconate. Maximum effects occurred at high concentrations. Combinations of agents were also examined, and the most effective mixture for stabilizing tubulin found was the combination of 1.0 M glutamate, 100 mM glucose 1-phosphate, 1 mM GTP and 0.5 mg/ml of albumin. No activity loss occurred over 48 h at 37.degree. C when tubulin was present at a concentration of 100 .mu.g/ml.