Comparative Genomics and Transcriptomics of Organohalide-Respiring Bacteria and Regulation of rdh Gene Transcription
Comparative Genomics and Transcriptomics of Organohalide-Respiring Bacteria and Regulation of rdh Gene Transcription
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DOI：
10.1007/978-3-662-49875-0_15
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
T. Kruse;H. Smidt;U. Lechner
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文献类型：
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作者：
T. Kruse;H. Smidt;U. Lechner
有机卤化物呼吸细菌基因组的比较，提高了我们的有机卤化物呼吸过程的遗传背景的理解。在本章中，专性和兼性有机卤化物呼吸细菌的还原脱卤酶编码基因的数量和辅助基因的数量和类型之间的显着差异进行了讨论，在不同的生活方式和进化方面。此外，辅助基因的假定功能进行了讨论，并提出了一个统一的命名。这些基因组也反映了合成或获得类咕啉辅因子的不同机制 还原脱卤酶 这与所观察到的各有机卤化物呼吸细菌的生长要求雅阁。基于微阵列的比较基因组学，转录组学和定量转录分析的价值，了解有机卤化物呼吸的生理和环境意义进行了讨论。还原性脱卤酶基因通常与编码转录调节因子的基因相关，转录调节因子可能参与感受卤代电子受体。两类调控因子在有机卤化物呼吸转录调控中的作用 已经被调查了多重抗生素抗性调节因子（马尔R）型调节因子对麦卡氏脱卤球菌还原性脱卤酶基因的转录具有负调控作用。在Desulfitobacteriumsanniense中，cAMP受体蛋白/富马酸盐和硝酸盐还原（CRP/FNR）调节剂CprK ，激活还原脱卤酶的转录 基因.邻氯酚作为效应物的分子机制已经阐明，以及如何通过诱导结构变化，导致调节剂的DNA结合。
Comparison of the genomes of organohalide-respiring bacteria has improved our understanding of the genetic background of the organohalide respiration process. In this chapter the remarkable differences between obligate and facultative organohalide-respiring bacteria in the number of reductive dehalogenase-encoding genes and the numbers and types of accessory genes are discussed in relation to different lifestyles and evolutionary aspects. Furthermore, the putative function of accessory genes is discussed and a unifying nomenclature is proposed. The genomes also reflect distinct mechanisms for the synthesis or acquisition of the corrinoid cofactors of reductive dehalogenases , which are well in accord with the observed growth requirements of the respective organohalide-respiring bacteria. The value of microarray-based comparative genomics, transcriptomics, and quantitative transcription analyses for understanding the physiology and environmental significance of organohalide respiration is discussed. The reductive dehalogenase genes are in general associated with genes encoding transcriptional regulators, which are likely involved in sensing the halogenated electron acceptors. The role of two types of regulators in transcriptional regulation of organohalide respiration has been investigated. A multiple antibiotic resistance regulator (MarR)-type regulator was shown to regulate negatively the transcription of reductive dehalogenase genes inDehalococcoidesmccartyi. InDesulfitobacteriumhafniense, the cAMP receptor protein/fumarate and nitrate reduction (CRP/FNR) regulator, CprK , activates transcription of reductive dehalogenase genes. The molecular mechanism of howortho-chlorophenols act as effectors has been elucidated and how, through the induction of structural changes, they lead to DNA binding of the regulator.