Increased expression of neuronal nitric oxide synthase in bladder afferent cells in the lumbosacral dorsal root ganglia after chronic bladder outflow obstruction.
Increased expression of neuronal nitric oxide synthase in bladder afferent cells in the lumbosacral dorsal root ganglia after chronic bladder outflow obstruction.
复制标题
慢性膀胱流出道梗阻后腰骶背根神经节膀胱传入细胞中神经元一氧化氮合酶的表达增加。
DOI：
10.1016/j.brainres.2003.12.016
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Vizzard,MargaretA
中科院分区：
文献类型：
--
作者：
Zvara,Peter;Folsom,JeffreyB;KlimentJr,Jan;Dattilio,AbbeyL;Moravcikova,Adriana;Plante,MarkK;Vizzard,MargaretA
一氧化氮 (NO) 是自主反射通路中的一种神经递质，在下尿路的功能性神经调节中发挥作用。神经元一氧化氮合酶 (nNOS) 水平上调，神经元一氧化氮合酶 (nNOS) 是负责 NO 合成的酶系统，已在膀胱炎、脊髓损伤后膀胱/括约肌协同失调和脑梗塞后膀胱过度活动等疾病的排尿反射的外周、脊髓和脊髓上段中发现其水平上调。这些观察结果表明，NO 可能在膀胱过度活动的发生中发挥作用。在这项研究中，评估了雄性和雌性大鼠膀胱流出道梗阻（BOO）后膀胱传入神经元和脊髓神经元中的 nNOS 免疫反应性（IR）。通过在近端尿道周围放置管腔缩小结扎线来诱导慢性 BOO。梗阻手术或假手术后六周，通过清醒膀胱测压法评估膀胱功能。通过将坚蓝注射到膀胱平滑肌中进行逆行神经元标记，鉴定 L1、L2、L6 和 S1 背根神经节 (DRG) 中的膀胱传入神经元。随后评估了 DRG 和相关脊髓节段的膀胱传入神经元中 nNOS-IR 的差异分布。表达 nNOS-IR 的膀胱传入神经元的百分比在 L6（男性中为 1.8 倍，女性中为 1.9 倍）和 S1（男性中为 2.8 倍，女性中为 5.3 倍）DRG 中增加。相比之下，在所检查的任何脊髓节段中均未观察到神经元或纤维分布中 nNOS-IR 的变化。
Nitric oxide (NO), a neurotransmitter in autonomic reflex pathways, plays a role in functional neuroregulation of the lower urinary tract. Upregulation of the levels of neuronal nitric oxide synthase (nNOS), the enzyme system responsible for NO synthesis, has been documented in the peripheral, spinal and supraspinal segments of the micturition reflex in diseases such as cystitis, bladder/sphincter dyssynergia following spinal cord injury and bladder overactivity after cerebral infarction. These observations suggest that NO might play a role in the development of bladder overactivity. In this study, nNOS-immunoreactivity (IR) was evaluated in bladder afferent and spinal neurons following bladder outflow obstruction (BOO) in male and female rats. Chronic BOO was induced by placing lumen reducing ligatures around the proximal urethra. Six weeks following the obstructive or sham surgery, bladder function was evaluated by awake cystometry. Bladder afferent neurons in L1, L2, L6 and S1 dorsal root ganglia (DRG) were identified by retrograde neuronal labeling with injection of Fast Blue into the bladder smooth muscle. A differential distribution of nNOS-IR was subsequently evaluated in bladder afferent neurons in the DRG and in the associated spinal cord segments. The percentage of bladder afferent neurons expressing nNOS-IR was increased in L6 (1.8-fold in males and 1.9-fold in females) and S1 (2.8-fold in males and 5.3-fold in females) DRG. In contrast, no changes in nNOS-IR in neurons or fiber distribution were observed in any spinal cord segments examined.