Stimulatory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on insulin and glucagon release from the isolated perfused rat pancreas.
Stimulatory effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on insulin and glucagon release from the isolated perfused rat pancreas.
复制标题
垂体腺苷酸环化酶激活多肽（PACAP）对离体灌注大鼠胰腺释放胰岛素和胰高血糖素的刺激作用。
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
K. Yamashita
中科院分区：
文献类型：
--
作者：
C. Yokota;K. Kawai;S. Ohashi;Y. Watanabe;S. Suzuki;K. Yamashita
采用大鼠胰腺灌注法研究垂体腺苷酸环化酶激活多肽（PACAP）对胰腺激素释放的影响。在灌注葡萄糖浓度为5.5 mmol/l时，0.1 nmol/l PACAP27显著刺激胰岛素和胰高血糖素的释放。刺激程度呈剂量依赖性。与2.8、5.5和8.3 mmol/l灌注葡萄糖浓度相比，胰岛素释放的刺激明显依赖于灌注葡萄糖浓度。在灌注葡萄糖浓度为5.5 mmol/l时，PACAP38对胰岛素释放的促进作用大于PACAP27，而在8.3 mmol/l时，PACAP38对胰岛素释放的促进作用小于PACAP27。两种肽之间胰高血糖素和cAMP的释放没有差异。PACAP对胰岛素和胰高血糖素释放的刺激作用分别被等摩尔浓度和十倍浓度的生长抑素完全消除。PACAP的胰岛激素释放的这些有效作用的生理学意义必须通过进一步的研究来阐明。
Rat pancreas perfusion was performed to study the effects of pituitary adenylate cyclase activating polypeptide (PACAP) on pancreatic hormone release. Under the perfusate glucose concentration of 5.5 mmol/l, 0.1 nmol/l PACAP27 significantly stimulated both insulin and glucagon release. The degree of stimulation was in a dose dependent manner. The stimulation of insulin release was clearly dependent on the perfusate glucose concentration, when compared with 2.8, 5.5 and 8.3 mmol/l. The potency of PACAP38 on the stimulation of insulin release was greater than that of PACAP27 at 5.5 mmol/l of perfusate glucose concentration, but not at 8.3 mmol/l. No differences for glucagon and cAMP release were found between the two peptides. PACAP's stimulatory effects on insulin and glucagon release were completely abolished by an equimolar and ten times lower concentration of somatostatin, respectively. The physiologic significance of these potent effects of PACAP's islet hormones release must be clarified by further studies.