INITIAL-STAGES OF TRYPSINIZED CELL-CULTURE OF CARDIAC MYOBLASTS - ULTRASTRUCTURAL DATA
INITIAL-STAGES OF TRYPSINIZED CELL-CULTURE OF CARDIAC MYOBLASTS - ULTRASTRUCTURAL DATA
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DOI：
10.1016/0022-2828(80)90111-x
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发表时间：
1980-01-01
影响因子：
5
通讯作者：
MALET, P
中科院分区：
文献类型：
--
作者：
PERISSEL, B;CHARBONNE, F;MALET, P
胰蛋白酶在铺板之前用于分离心肌细胞的作用是明确的。鉴定了接种沉积物中的不同细胞类型。在培养物中，新生大鼠的心肌细胞在器官细胞解离后似乎经历了两个连续的阶段。直至培养 24 小时，观察到细胞结构紊乱的阶段，其特征是成肌细胞内部组织出现紊乱并形成新结构。这个解体阶段之后是重组和分化阶段，在此期间完成某些细胞器的渐进和伴随成熟（粘附筋膜的进化、肌丝轨迹的设置和肌瘤发生）。细胞分化从培养 48 小时开始增加，在此阶段整个培养物有节奏地收缩。
The action of trypsin used to separate myocardial cells before their plating is specified. Different cellular types in the seeding deposit are identified. In culture the myocardial cells of new-born rats appear to undergo 2 successive stages following the cell dissociation of the organ. Up to the 24th h of culture, a stage of disorganization of cellular structures is observed, characterized by the appearance of disorder in the internal organization of the myoblasts and by the formation of new structures. This stage of disorganization is followed by a stage of reorganization and differentiation, during which the progressive and concomitant maturation of certain organelles is accomplished (evolution of fasciae adhaerentes, the setting of the trails of myofilaments and sarcomerogenesis). The cellular differentiation increases from 48 h of culture, a stage during which the whole culture rhythmically contracts.