Characterization of heat shock cognate protein 70 gene and its differential expression in response to thermal stress between two wing morphs of Nilaparvata lugens (Stal)
Characterization of heat shock cognate protein 70 gene and its differential expression in response to thermal stress between two wing morphs of Nilaparvata lugens (Stal)
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DOI：
10.1016/j.cbpa.2016.05.009
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发表时间：
2016-09-01
影响因子：
2.3
通讯作者：
Zhou, Qiang
中科院分区：
文献类型：
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作者：
Lu, Kai;Chen, Xia;Zhou, Qiang
先前的研究表明，两种翅型褐飞虱（亚洲最严重的水稻害虫）之间的耐热性存在差异。为了揭示两种翅膀形态之间耐热能力差异的分子调控机制，克隆了编码热休克同源蛋白 70 (Hsc70) 的全长转录本，并分析了其在温度梯度下的表达模式。结果表明，在32℃至38℃热激后，大翅类中N1Hsc70的表达水平急剧增加，而短翅类中N1Hsc70转录本在不同温度胁迫条件下保持恒定。此外，40℃热激恢复1h和2h后，大翅类中N1Hsc70的表达显着高于短翅类。冷激条件下短翅目和大翅目N1Hsc70 mRNA表达无显着差异。因此，N1Hsc70确实是褐飞虱短翅目中Hsp70家族的组成型表达成员，而在大翅目中则为热诱导型。此外，注射N1Hsc70 dsRNA的两种变体在40℃热休克或0℃冷休克1小时后存活率显着降低。这些结果表明，N1Hsc70的上调可能与热阻有关，并且NIHsc70在大翅类中更有效的诱导表达可以促进温度胁迫条件下更大的热耐受性。 (C) 2016 Elsevier Inc. 保留所有权利。
Previous studies have demonstrated differences in thermotolerance between two wing morphs of Nilaparvata lugens, the most serious pest of rice across the Asia. To reveal the molecular regulatory mechanisms underlying the differential thermal resistance abilities between two wing morphs, a full-length of transcript encoding heat shock cognate protein 70 (Hsc70) was cloned, and its expression patterns across temperature gradients were analyzed. The results showed that the expression levels of N1Hsc70 in macropters increased dramatically after heat shock from 32 to 38 degrees C, while N1Hsc70 transcripts in brachypters remained constant under different temperature stress conditions. In addition, N1Hsc70 expression in the macropters was significantly higher than that in brachypters at 1 and 2 h recovery from 40 degrees C heat shock. There was no significant difference in N1Hsc70 mRNA expression between brachypters and macropters under cold shock conditions. Therefore, N1Hsc70 was indeed a constitutively expressed member of the Hsp70 family in brachypters of N. lugens, while it was heat-inducible in macropters. Furthermore, the survival rates of both morphs injected with N1Hsc70 dsRNA were significantly decreased following heat shock at 40 degrees C or cold shock at 0 degrees C for 1 h. These results suggested that the up-regulation of N1Hsc70 is possibly related to the thermal resistance, and the more effective inducement expression of NIHsc70 in macropters promotes a greater thermal tolerance under temperature stress conditions. (C) 2016 Elsevier Inc. All rights reserved.