Immunohistochemical expression of PTEN in normal, hyperplastic and malignant endometrium and its correlation with hormone receptors, bcl-2, bax, and apoptotic index
Immunohistochemical expression of PTEN in normal, hyperplastic and malignant endometrium and its correlation with hormone receptors, bcl-2, bax, and apoptotic index
复制标题
DOI：
10.1016/j.prp.2007.01.003
复制
发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Ciris, Metin
中科院分区：
文献类型：
--
作者：
Kapucuoglu, Nilgun;Aktepe, Fatma;Ciris, Metin
PTEN 是一种抑癌基因，在 I 型子宫内膜样子宫内膜癌 (EEC) 中频繁突变，参与细胞增殖、分化和凋亡的控制。在本研究中，我们旨在评估EEC、其前体病变增生和周期性子宫内膜中PTEN表达与雌激素、孕激素受体（PR）、其他凋亡相关蛋白（例如bcl-2和bax）以及凋亡指数（AI）之间的关系。我们还评估了 PTEN 表达与临床病理参数之间的关系。 PTEN，雌激素受体（ER），PR，。通过免疫组织化学方法评估 bcl-2 和 bax 表达，并在 23 个周期性子宫内膜、37 个增生性子宫内膜以及 35 个 EEC 的苏木精和伊红 (HE) 染色玻片中评估 AI。周期性子宫内膜中的 PTEN 表达高于癌中的表达 (p < 0.05)。非典型增生中 PTEN 表达水平显着高于 EEC，但非典型复合性增生 (ACH) 与 EEC 之间以及增生之间无差异。在癌症中，分级与 PTEN 表达呈负相关（r = -0.338，p = 0.047）。总之，我们推测 PTEN 参与子宫内膜肿瘤发生的早期阶段，并且可以推测，随着癌中分化的丧失，PTEN 表达的减少可能导致具有更具侵袭性表型的肿瘤的出现。 (c) 2007 年爱思唯尔有限公司。版权所有。
PTEN is a tumor supressor gene that is frequently mutated in type I endometrioid endometrial carcinomas (EECs), and is involved in the control of cell proliferation, differentiation, and apoptosis. In this study, we aimed to assess the relationship between PTEN expression and estrogen, progesterone receptors (PRs), other apoptosis-related proteins, such as bcl-2 and bax, and apoptotic index (AI) in EEC, its precursor lesion hyperplasia, and cyclical endometrium. We also evaluated the relationship between PTEN expression and clinicopathologic parameters. PTEN, estrogen receptor (ER), PR,. and bcl-2 and bax expressions were evaluated immunohistochemically, and AI was evaluated in hematoxylin and eosin (HE)-stained slides in 23 cyclical and 37 hyperplastic endometria, and in 35 EECs. PTEN expression was higher in cyclical endometrium than in the carcinomas (p < 0.05). The PTEN expression level was significantly higher in non-atypical hyperplasias than in EEC, but there were no differences between atypical complex hyperplasia (ACH) and EEC and between hyperplasias. In the carcinomas, there was a negative correlation between grade and PTEN expression (r = -0.338, p = 0.047). In conclusion, we presume that PTEN is involved in the early phases of endometrial tumorigenesis, and it can be speculated that decreased PTEN expression with loss of differentiation in carcinoma can contribute to the emergence of tumors with a more aggressive phenotype. (c) 2007 Elsevier GmbH. All rights reserved.