Metabolic potential of uncultured bacteria and archaea associated with petroleum seepage in deep-sea sediments
Metabolic potential of uncultured bacteria and archaea associated with petroleum seepage in deep-sea sediments
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DOI：
10.1038/s41467-019-09747-0
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发表时间：
2019-04-18
影响因子：
16.6
通讯作者：
Hubert, Casey R. J.
中科院分区：
文献类型：
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作者：
Dong, Xiyang;Greening, Chris;Hubert, Casey R. J.
由于缺乏深海海底的微生物基因组和分离物，人们对这个巨大栖息地的生态知之甚少。在此，我们研究了墨西哥湾东部三个深水海底石油渗漏（水深3公里）中微生物群落的能量和碳获取策略。散弹枪宏基因组分析表明，每种沉积物中都有不同的趋化异养生物和趋化养生物群落。我们恢复了82个宏基因组组装的基因组，隶属于21个不同的古细菌和细菌门。多个基因组编码用于脂肪族和芳香族化合物厌氧氧化的酶，包括候选的厌氧菌门、Aminicenantes、TA06和Bathyarchaeota。微生物相互作用预计是由醋酸和氢分子驱动的。这些发现得到了沉积物地球化学、代谢组学和热力学模型的支持。综上所述，我们推断，经历热生烃输入的深海沉积物具有系统发育和功能多样化的群落，可能通过厌氧烃、乙酸和氢代谢维持。
The lack of microbial genomes and isolates from the deep seabed means that very little is known about the ecology of this vast habitat. Here, we investigate energy and carbon acquisition strategies of microbial communities from three deep seabed petroleum seeps (3 km water depth) in the Eastern Gulf of Mexico. Shotgun metagenomic analysis reveals that each sediment harbors diverse communities of chemoheterotrophs and chemolithotrophs. We recovered 82 metagenome-assembled genomes affiliated with 21 different archaeal and bacterial phyla. Multiple genomes encode enzymes for anaerobic oxidation of aliphatic and aromatic compounds, including those of candidate phyla Aerophobetes, Aminicenantes, TA06 and Bathyarchaeota. Microbial interactions are predicted to be driven by acetate and molecular hydrogen. These findings are supported by sediment geochemistry, metabolomics, and thermodynamic modelling. Overall, we infer that deep-sea sediments experiencing thermogenic hydrocarbon inputs harbor phylogenetically and functionally diverse communities potentially sustained through anaerobic hydrocarbon, acetate and hydrogen metabolism.