Evaluation of a Rapid Enzyme-Linked Immunosorbent Assay for Diagnosis of Strongyloidiasis
Evaluation of a Rapid Enzyme-Linked Immunosorbent Assay for Diagnosis of Strongyloidiasis
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DOI：
10.1128/jcm.02364-09
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发表时间：
2010-05-01
影响因子：
9.4
通讯作者：
Hennequin, C.
中科院分区：
文献类型：
--
作者：
Bon, B.;Houze, S.;Hennequin, C.
由于缺乏敏感性和苛刻的技术，利用粪便检查诊断类圆线虫病仍然不能令人满意。在这项工作中，我们研究了抗类圆线虫IgG酶免疫测定（EIA）的价值，使用回顾性收集的207份血清，分别来自确诊为类圆线虫病（n = 57）、其他蠕虫感染（n = 46）、嗜酸性粒细胞增多症（n = 54）、和热带旅行后的消化紊乱（n = 30）和来自20个阴性对照。通过遵循受试者工作特征（ROC）曲线分析，可以优化测试以达到91.2%的灵敏度和93.3%的特异性，92.8%的患者正确分类。考虑到在我们自己的实验室诊断的类圆线虫病的发病率，阴性预测值计算为99.9%。总之，这种测试是非常快速，易于执行，可能是有价值的诊断类圆线虫病的情况下，感染是未透露的寄生虫粪便检查和患者的风险，严重的临床形式，如患者接受免疫抑制治疗。
Diagnosis of strongyloidiasis using stool examination remains unsatisfactory due to the lack of sensitivity and fastidious techniques. In this work, we investigated the value of an anti-Strongyloides IgG enzyme immunoassay (EIA), using a panel of 207 sera retrospectively collected from patients with definitive diagnoses of strongyloidiasis (n = 57), other helminthic infections (n = 46), eosinophilia without parasitic infection diagnosis (n = 54), and digestive disturbances following a tropical journey (n = 30) and from 20 negative controls. By following a receiver operating characteristic (ROC) curve analysis, it was possible to optimize the test to reach a sensitivity of 91.2% and a specificity of 93.3%, with 92.8% of patients correctly classified. Considering the incidence of strongyloidiasis diagnosed in our own laboratory, the negative predictive value was calculated at 99.9%. In conclusion, this test is very rapid and easy to perform and may be valuable for diagnosis of strongyloidiasis both in cases where the infection is unrevealed by a parasitological stool examination and in patients at risk for severe clinical forms, such as patients receiving immunosuppressive therapy.