Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri-implant or junctional epithelium of rat gingivae
Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri-implant or junctional epithelium of rat gingivae
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DOI：
10.1034/j.1600-0501.2002.130303.x
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发表时间：
2002-06-01
影响因子：
4.3
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Ikeda, H;Shiraiwa, M;Tanaka, T
将辣根过氧化物酶（HRP）示踪剂以5、25和50 mg/ml的浓度应用于种植体或天然牙的龈沟，以研究种植体周围上皮（PIE）和结合上皮（JE）的封闭能力;在电子显微镜下观察HRP渗透的程度。在拔除大鼠上颌左第一磨牙后立即（立即植入）或2周（延迟植入）植入Ti-6Al-4V种植体。右侧磨牙的JE用作对照。尽管未脱钙切片的整个PIE似乎附着在植入物表面，但电子显微镜下，PIE冠状中间区域的内部基底层（IBL）和半桥粒缺乏。HRP在PIE细胞间的细胞间隙有广泛的细胞外沉积，HRP在一定程度上被外基底层的透明层和致密层以及基底细胞连接所阻断。HRP在PIE下的结缔组织中检测到，而在JE下的结缔组织中未检测到。在细胞内，HRP可见于PIE细胞和JE细胞的囊泡和颗粒中。这些在PIE细胞中的数量比在JE细胞中少。即刻和延迟植入的结果之间没有差异。结果表明，在IBL的缺陷允许HRP从龈沟渗透到结缔组织下的PIE，并建议，内吞能力的PIE细胞是劣于JE细胞。
Horseradish peroxidase (HRP) tracer was applied to the gingival sulcus of implants or natural teeth at 5, 25, or 50 mg/ml to investigate the sealing capacities of the periimplant epithelium (PIE) and junctional epithelium (JE); the extent of HRP penetration was observed under electron microscopy. A Ti-6Al-4V implant was inserted either immediately (immediate implantation) or 2 weeks (delayed implantation) after extraction of the maxillary left first molar of rats. The JE of the right molar was used as a control. Although the whole PIE of undecalcified sections appeared to be attached to the implant surface, electron microscopically, the internal basement lamina (IBL) and hemidesmosomes were deficient in the coronal-middle region of the PIE. There were extensive extracellular deposits of HRP in the intercellular spaces between PIE cells, and HRP was blocked to some extent by the lamina lucida and lamina densa of the external basal lamina and basal cell junction. HRP was detected in the connective tissue under the PIE, but was not found in the connective tissue under the JE. Intracellularly, HRP was found in the vesicles and granules of PIE cells and JE cells. These were fewer in number in PIE cells than in JE cells. There were no differences between the findings for immediate and delayed implantation. The results indicate that a deficiency in the IBL permitted penetration of HRP from the gingival sulcus into the connective tissue under the PIE, and suggest that the endocytotic capacity of PIE cells is inferior to that of JE cells.