TISSUE EFFECTS OF IRON-DEFICIENCY IN THE RAT
TISSUE EFFECTS OF IRON-DEFICIENCY IN THE RAT
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DOI：
10.1016/0304-4165(83)90061-2
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BROWN, EB
中科院分区：
文献类型：
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作者：
MCKAY, RH;HIGUCHI, DA;BROWN, EB
对断奶时喂食缺铁饮食的雄性 Wistar 大鼠的各种后腿肌肉、快肌红肌和白肌以及心脏和肝脏进行了琥珀酸脱氢酶和线粒体 3-磷酸甘油脱氢酶活性、Fe、细胞色素 c 和肌红蛋白的测量。给大鼠喂食相同的饮食，并腹腔注射 20 毫克铁。 Fe-葡聚糖（Imferon）作为对照。对于铁补充研究，贫血大鼠 (Hb < 7 g/dl) 单次注射 10 mg Fe (Imferon)，并跟踪上述参数变化的时间过程，直至注射后 22 天。大多数缺铁肌肉的铁浓度下降至.apprx。控制为35%，心脏为60%，肝脏为13%。补充后，所有组织的 Fe 浓度在 4 天后显着增加。虽然细胞色素 c 和肌红蛋白的水平接近肌肉中的铁水平，但它们在心脏中没有显着变化。肌肉中琥珀酸脱氢酶活性大幅下降，降至对照的 10-30%；补充后，活性显着增加。线粒体甘油-3-磷酸脱氢酶活性在缺铁组织中仅表现出很小的变化。
Measurements of succinate dehydrogenase and mitochondrial glycerol-3-phosphate dehydrogenase activities, Fe, cytochrome c and myoglobin, were made on various hind-leg muscles, fast-twitch red and white muscle and heart and liver of male Wistar rats fed an Fe-deficient diet on weaning. Rats fed the same diet and given 20 mg Fe i.p. as Fe-dextran (Imferon) served as controls. For Fe-repletion studies anemic rats (Hb < 7 g/dl) were given a single injection of 10 mg Fe (Imferon) and the time course of change in the above parameters was followed up to 22 days after injection. The Fe concentration of most Fe-deficient muscles dropped to .apprx. 35% of control, the heart to 60% and liver to 13%. On repletion, the Fe concentration of all tissues increase significantly by 4 days. While the levels of cytochrome c and myoglobin approximated the Fe levels in muscle, they did not change significantly in the heart. Succinate dehydrogenase activity dropped profoundly in muscle, to 10-30% of control; on repletion, the activity increased significantly. Mitochondrial glycerol-3-phosphate dehydrogenase activity showed only small changes in Fe-deficient tissues.