Vessel tortuousity and reduced vascularization in the fetoplacental arterial tree after maternal exposure to polycyclic aromatic hydrocarbons
Vessel tortuousity and reduced vascularization in the fetoplacental arterial tree after maternal exposure to polycyclic aromatic hydrocarbons
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DOI：
10.1152/ajpheart.00510.2010
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发表时间：
2011-02-01
影响因子：
4.8
通讯作者：
Sled, John G.
中科院分区：
文献类型：
--
作者：
Rennie, Monique Y.;Detmar, Jacqui;Sled, John G.
Rennie MY，Detmar J，Whiteley KJ，Yang J，Jurisicova A，Adamson SL，Sled JG.母体暴露于多环芳烃后胎儿胎盘动脉树血管迂曲和血管化减少。美国生理学杂志心脏循环生理学300：H675-H684，2011年。首次发表于2010年12月10日; doi：10.1152/ajpheart.00510.2010.-多环芳烃是一种普遍存在的环境污染物，也是卷烟中的主要有毒物质。妇女在怀孕前经常接触到多环芳烃，通常是通过怀孕前吸烟。为了确定如何影响胎儿胎盘血管的胎盘，孕前暴露于多环芳烃雌性小鼠怀孕前，灌注胎儿胎盘动脉树与X射线造影剂，并成像的血管离体微计算机断层扫描（micro-CT）在胚胎第15.5天。自动血管分割和流量计算显示，在对照树中，< 40个绒毛膜板血管（直径> 180 μ m）产生类似于1，300个胎盘内动脉（50-180 μ m），预测动脉血管阻力为0.37 +/-0.04（-1）。mmHg.s.mu PAH暴露增加绒毛板血管的血管曲率，并显着增加树的迂曲率。胎盘内动脉减少了17%，主要是由于小动脉大小（50-100 μ m）的血管数量减少了27%。绒毛膜血管的数量，树的深度或跨度，直径缩放系数，或段的长度与直径比没有变化。多环芳烃暴露导致一棵树具有相似的大小和二叉分支结构，但一个是相对稀疏，使动脉血管阻力增加了30%。假设压力梯度相同，血流量将降低19%。低血流可能导致观察到的胎儿体重减少23%。使用显微CT成像和自动血管分割分析，实现了对PAH暴露对发育中动脉树的具体影响的新见解。
Rennie MY, Detmar J, Whiteley KJ, Yang J, Jurisicova A, Adamson SL, Sled JG. Vessel tortuousity and reduced vascularization in the fetoplacental arterial tree after maternal exposure to polycyclic aromatic hydrocarbons. Am J Physiol Heart Circ Physiol 300: H675-H684, 2011. First published December 10, 2010; doi: 10.1152/ajpheart.00510.2010.-Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants and the main toxicants found in cigarettes. Women are often exposed to PAHs before pregnancy, typically via prepregnancy smoking. To determine how prepregnancy exposure affects the fetoplacental vasculature of the placenta, we exposed female mice to PAHs before conception, perfused the fetoplacental arterial trees with X-ray contrast agent, and imaged the vasculature ex vivo by microcomputed tomography (micro-CT) at embryonic day 15.5. Automated vascular segmentation and flow calculations revealed that in control trees, < 40 chorionic plate vessels (diameter > 180 mu m) gave rise to similar to 1,300 intraplacental arteries (50-180 mu m), predicting an arterial vascular resistance of 0.37 +/- 0.04 mmHg.s.mu l(-1). PAH exposure increased vessel curvature of chorionic plate vessels and significantly increased the tortuousity ratio of the tree. Intraplacental arteries were reduced by 17%, primarily due to a 27% decrease in the number of arteriole-sized (50-100 mu m) vessels. There were no changes in the number of chorionic vessels, the depth or span of the tree, the diameter scaling coefficient, or the segment length-to-diameter ratio. PAH exposure resulted in a tree with a similar size and dichotomous branching structure, but one that was comparatively sparse so that arterial vascular resistance was increased by 30%. Assuming the same pressure gradient, blood flow would be 19% lower. Low flow may contribute to the 23% reduction observed in fetal weight. New insights into the specific effects of PAH exposure on a developing arterial tree were achieved using micro-CT imaging and automated vascular segmentation analysis.