Understanding microstructure of the brain by comparison of neurite orientation dispersion and density imaging (NODDI) with transparent mouse brain.
Understanding microstructure of the brain by comparison of neurite orientation dispersion and density imaging (NODDI) with transparent mouse brain.
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DOI：
10.1177/2058460117703816
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发表时间：
2017-04
影响因子：
1.1
通讯作者：
Aoki S
中科院分区：
文献类型：
--
作者：
Sato K;Kerever A;Kamagata K;Tsuruta K;Irie R;Tagawa K;Okazawa H;Arikawa-Hirasawa E;Nitta N;Aoki I;Aoki S
神经突方向弥散和密度成像（NODDI）是一种扩散磁共振成像（MRI）技术，具有可视化大脑微观结构的潜力。革命性的组织学方法，使小鼠大脑透明最近已经开发出来，但NODDI的验证，这些方法还没有报道。证实NODDI与组织学在脑神经突密度和方向分散方面的一致性。用7-T MRI扫描仪获得thy-1黄色荧光蛋白小鼠的全脑扩散MRI，之后从同一小鼠产生透明脑切片。从MR图像计算的NODDI参数，包括细胞内体积分数（维克）和取向分散指数（ODI），与组织学结果进行比较。比较前连合和海马中含有交叉纤维（交叉区）和平行纤维（平行区）的区域之间的神经突密度、维克和ODI，并评估纤维密度和维克之间的相关性。在前连合和海马中，交叉区的ODI显著高于平行区（分别为P = 0.0247，P = 0.00022）。神经突密度显示出类似的趋势，但仅在海马中存在显著差异（P = 7.91E-07）。神经突密度与维克无明显相关性。通过组织学验证NODDI用于量化神经突的取向分散。这些结果表明，ODI是一个合适的指标，了解在体脑的微观结构。
Neurite orientation dispersion and density imaging (NODDI) is a diffusion magnetic resonance imaging (MRI) technique with the potential to visualize the microstructure of the brain. Revolutionary histological methods to render the mouse brain transparent have recently been developed, but verification of NODDI by these methods has not been reported. To confirm the concordance of NODDI with histology in terms of density and orientation dispersion of neurites of the brain. Whole brain diffusion MRI of a thy-1 yellow fluorescent protein mouse was acquired with a 7-T MRI scanner, after which transparent brain sections were created from the same mouse. NODDI parameters calculated from the MR images, including the intracellular volume fraction (Vic) and the orientation dispersion index (ODI), were compared with histological findings. Neurite density, Vic, and ODI were compared between areas of the anterior commissure and the hippocampus containing crossing fibers (crossing areas) and parallel fibers (parallel areas), and the correlation between fiber density and Vic was assessed. The ODI was significantly higher in the crossing area compared to the parallel area in both the anterior commissure and the hippocampus (P = 0.0247, P = 0.00022, respectively). Neurite density showed a similar tendency, but was significantly different only in the hippocampus (P = 7.91E−07). There was no significant correlation between neurite density and Vic. NODDI was verified by histology for quantification of the orientation dispersion of neurites. These results indicate that the ODI is a suitable index for understanding the microstructure of the brain in vivo.