A family of ubiquitin-like proteins binds the ATPase domain of Hsp-70-like Stch
A family of ubiquitin-like proteins binds the ATPase domain of Hsp-70-like Stch
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DOI：
10.1016/s0014-5793(00)01135-2
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发表时间：
2000-02-11
期刊：
影响因子：
3.5
通讯作者：
Rose, MD
中科院分区：
文献类型：
--
作者：
Kaye, FJ;Modi, S;Rose, MD
我们已经分离出两个人的泛素样（UbL）蛋白结合到一个短肽内的ATP酶结构域的Hsp 70样Stch蛋白。Chap 1是酵母Dsk 2基因的重复同源物，其是通过细胞周期的G2/M期转运所需的，并且人全长cDNA的表达恢复了活力并抑制了dsk 2 Delta rad 23 Delta酿酒酵母突变体的G2/M停滞表型。Chap 2是爪蟾镰刀的同源物，爪蟾镰刀是卵提取物中收割者诱导的细胞凋亡的重要组成部分。虽然S-末端UbL结构域对于Stch结合不是必需的，Chap 1/Dsk 2包含Sti 1样重复序列，其需要与Stch结合，并且在Hsp 70结合蛋白Hip和p60/Sti 1/Hop中也是保守的。这些发现扩展了Hsp 70成员和编码UbL序列的基因之间的关联，并表明Hsp 70样ATP酶家族在调节细胞周期和细胞死亡事件中的更广泛作用，（C）2000欧洲生物化学学会联合会。
We have isolated two human ubiquitin-like (UbL) proteins that bind to a short peptide within the ATPase domain of the Hsp70-like Stch protein. Chap1 is a duplicated homologue of the yeast Dsk2 gene that is required for transit through the G2/M phase of the cell cycle and expression of the human full-length cDNA restored viability and suppressed the G2/M arrest phenotype of dsk2 Delta rad23 Delta Saccharomyces cerevisiae mutants. Chap2 is a homologue for Xenopus scythe which is an essential component of reaper-induced apoptosis in egg extracts, While the S-terminal UbL domains were not essential for Stch binding, Chap1/Dsk2 contains a Sti1-like repeat sequence that is required far binding to Stch and is also conserved in the Hsp70 binding proteins, Hip and p60/Sti1/Hop. These findings extend the association between Hsp70 members and genes encoding UbL sequences and suggest a broader role for the Hsp70-like ATPase family in regulating cell cycle and cell death events, (C) 2000 Federation of European Biochemical Societies.