Cloning, characterization, and possible origin of the Prevotella loescheii dnaK homolog.
Cloning, characterization, and possible origin of the Prevotella loescheii dnaK homolog.
复制标题
洛氏普雷沃氏菌 dnaK 同源物的克隆、表征和可能来源。
DOI：
10.1007/s0028403313
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发表时间：
2001
影响因子：
2.6
通讯作者：
Schneider,R
中科院分区：
文献类型：
--
作者：
Manch-Citron,JN;Shahani,PJ;Schneider,R
热休克蛋白在细菌生存和对环境应激的反应中发挥着重要作用。我们克隆了普氏菌 HSP70 同源物 (dnaK)，并表征了编码序列、调控区域以及与其他细菌的进化关系。预测由 theP 编码的蛋白质。 loescheii dnaKhomolog（开放阅读框 ORF-1）和两个下游编码区 ORF-2 和 ORF-3 与牙龈卟啉单胞菌 dnaK 区域的 ORF-4 (dnaK)、ORF-5 和 ORF-6 编码的蛋白质高度同源。 ThednaKpromoter 类似于其他 HSP（热休克蛋白）启动子。由ORF-2编码的预测蛋白质的比对显示与来自转座子Tn4555的脆弱拟杆菌tnpA基因显着同源，而ORF-3蛋白质显示与B同源性。脆弱菌转座酶 (Tn5220) 和整合酶 (Tn4555) 蛋白。这表明类似转座的事件可能是这些基因在卟啉单胞菌和普雷沃菌之间转移的原因。
Heat shock proteins play an important role in bacterial survival and response to environmental stress. We cloned thePrevotella loescheiiHSP70 homolog (dnaK) and characterized the coding sequence, regulatory regions, and evolutionary relationships to other bacteria. Predicted proteins encoded by theP. loescheii dnaKhomolog (open reading frame ORF-1) and two downstream coding regions, ORF-2 and ORF-3, are highly homologous to the proteins encoded by ORF-4 (dnaK), ORF-5, and ORF-6 from thednaKregion ofPorphyromonas gingivalis. ThednaKpromoter resembles other HSP (heat shock protein) promoters. Alignment of the predicted protein encoded by ORF-2 showed significant homology to theBacteroides fragilis tnpAgene from the transposon Tn4555, whereas the ORF-3 protein showed homology toB. fragilistransposase (Tn5220) and integrase (Tn4555) proteins. This suggests a transposition-like event may be responsible for transfer of these genes betweenPorphyromonasandPrevotella.