GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression.
GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression.
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DOI：
10.1093/jxb/ern292
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发表时间：
2009
影响因子：
6.9
通讯作者：
Webb AA
中科院分区：
文献类型：
--
作者：
Gardner MJ;Baker AJ;Assie JM;Poethig RS;Haseloff JP;Webb AA
为了便于在发育和分离期间监测保卫细胞，筛选了704个GAL 4 GFP增强子捕获系的群体，并鉴定了具有保卫细胞GFP表达的四个单插入系和具有发育调节的保卫细胞GFP表达的一个单插入系。对T-DNA插入片段的位置、侧翼基因的表达以及T-DNA上游基因组DNA的启动子活性进行了表征。结果表明，至少一个系中的GFP表达模式是由于紧邻T-DNA上游的基因间DNA中的元件，而不是由于插入片段侧翼的基因的启动子的活性，并且为Dof元件参与调节保卫细胞基因表达提供了证据。进一步表明，GAL 4 GFP系可用于体外追踪保卫细胞材料的贡献，并且该方法用于评估使用两种保卫细胞分离方法获得的保卫细胞样品的纯度。
To facilitate the monitoring of guard cells during development and isolation, a population of 704 GAL4 GFP enhancer trap lines was screened and four single insert lines with guard cell GFP expression and one with developmentally-regulated guard cell GFP expression were identified. The location of the T-DNA inserts, the expression of the flanking genes, and the promoter activity of the genomic DNA upstream of the T-DNA were characterized. The results indicated that the GFP expression pattern in at least one of the lines was due to elements in the intergenic DNA immediately upstream of the T-DNA, rather than due to the activity of the promoters of genes flanking the insert, and provide evidence for the involvement of Dof elements in regulating guard cell gene expression. It is shown further that the GAL4 GFP lines can be used to track the contribution of guard cell material in vitro, and this method was used to assess the purity of guard cell samples obtained using two methods of guard cell isolation.
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影响因子：
7.4
作者：
Desikan, R;Hancock, JT;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
4.2
作者：
Baxter-Burrell, A;Chang, R;Bailey-Serres, J
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Bailey-Serres, J
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7.2
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Clough, SJ;Bent, AF
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Bent, AF
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48
作者：
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通讯作者：
Benfey, PN
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7.2
作者：
Lechtenberg, B;Schubert, D;Schmidt, R
通讯作者：
Schmidt, R