Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1
Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1
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DOI：
10.1007/s00122-002-0968-3
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发表时间：
2002-12-01
影响因子：
5.4
通讯作者：
Nishio, T
中科院分区：
文献类型：
--
作者：
Inaba, R;Nishio, T
确定了 20 个十字花科物种中 S 位点相关基因 SLR1 直系同源物的核苷酸序列，并与之前报道的 6 个物种的 SLR1 序列进行了比较。推导的氨基酸序列与甘蓝SLR1的同源性在66.0%~97.6%之间，与甘蓝SRK和SLR2的同源性分别低于62%和55%。在推导的氨基酸序列的多重比对中，起始甲硫氨酸的第180-190个氨基酸残基是高度可变的，该可变区对应于SLG和SRK的高变区I。基于推导的氨基酸序列的系统发育树显示了十字花科和萝卜亚科物种的SLR1直系同源物之间的密切关系。发现黑甘蓝SLR1与Sinapis arvensis和Diplotaxis siifolia属于同一进化枝，而其他芸苔属物种的序列与B. oleracea和Brassica rapa一起属于另一个进化枝。该系统发育树与之前报道的利用叶绿体 DNA 电泳带模式数据构建的树相似，但发现了细微的差异。根据SLR1的同义替换率，估计了十字花科物种之间SLR1直向同源物的多样化时间。讨论了SLR1的进化和功能以及十字花科植物的系统发育关系。
Nucleotide sequences of orthologs of the S-locus related gene, SLR1, in 20 species of Brassicaceae were determined and compared with the previously reported SLR1 sequences of six species. Identities of deduced amino-acid sequences with Brassica oleracea SLR1 ranged from 66.0% to 97.6%, and those with B. oleracea SRK and SLR2 were less than 62% and 55%, respectively. In multiple alignment of deduced amino-acid sequences, the 180-190th amino-acid residues from the initial methionine were highly variable, this variable region corresponding to hypervariable region I of SLG and SRK. A phylogenetic tree based on the deduced amino-acid sequences showed a close relationship of SLR1 orthologs of species in the Brassicinae and Raphaninae. Brassica nigra SLR1 was found to belong to the same clade as Sinapis arvensis and Diplotaxis siifolia, while the sequences of the other Brassica species belonged to another clade together with B. oleracea and Brassica rapa. The phylogenetic tree was similar to previously reported trees constructed using the data of electrophoretic band patterns of chloroplast DNA, though minor differences were found. Based on synonymous substitution rates in SLR1, the diversification time of SLR1 orthologs between species in the Brassicinae was estimated. The evolution and function of SLR1 and the phylogenetic relationship of Brassiceae plants are discussed.