Effects of testosterone on PPARγ and P450arom expression in polycystic ovary syndrome patients and related mechanisms
Effects of testosterone on PPARγ and P450arom expression in polycystic ovary syndrome patients and related mechanisms
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DOI：
10.26355/eurrev_201803_14559
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发表时间：
2018-03-01
影响因子：
3.3
通讯作者：
Wang, C. -L.
中科院分区：
文献类型：
--
作者：
He, Y.;Wang, C. -L.
目的：多囊卵巢综合征（PCOS）可导致女性排卵障碍和不孕。本研究招募PCOS患者，采用睾酮治疗，分析其对PCOS患者卵巢颗粒细胞核过氧化物酶体增殖激活受体γ (PPAR γ)和细胞色素P450芳香化酶（P450arom）表达的影响。患者与方法：PCOS和非PCOS患者分别给予睾酮1.0 nmol/L、10 nmol/L。采用qRT-PCR检测PPAR γ和P450arom的表达，Western blot检测蛋白表达情况。结果：实验组PPAR γ mRNA表达水平显著升高，P450arom表达水平显著降低（p < 0.05）。10 nmol/l睾酮处理显著提高PPAR γ mRNA和蛋白表达，降低P450arom表达（p < 0.05）。PPAR γ水平与睾酮浓度呈正相关，与P450arom表达呈负相关（p < 0.05）。结论：睾酮引起PCOS患者高雄激素微环境，与PPAR γ升高和p450 - arom降低相关。
OBJECTIVE: Polycystic ovary syndrome (PCOS) causes ovulation disorder and infertility in female patients. This study recruited PCOS patients and adopted testosterone therapy to analyze its effect on expression of nuclear peroxisome proliferation activated receptor gamma (PPAR gamma), and cytochrome P450 aromatase (P450arom) in ovary granular cells of patients. PATIENTS ANDMETHODS: PCOS and non-PCOS patients were treated with testosterone at 1.0 nmol/L, 10 nmol/L. qRT-PCR was used to detect the expressions of PPAR gamma and P450arom while Western blot was performed for the evaluation of protein expression.RESULTS: In the experimental group, the level of PPAR gamma mRNA was significantly elevated whilst the expression of P450arom was statistically decreased (p < 0.05). The treatment of 10 nmol/l testosterone significantly elevated PPAR gamma mRNA and protein expression, and decreased P450arom expression (p < 0.05). The level of PPAR gamma was positively correlated with testosterone concentration, and was negatively correlated with P450arom expression (p < 0.05).CONCLUSIONS: Testosterone causes hyperandrogenism microenvironment of PCOS patients, with a correlated increase of PPAR gamma and reduction of P450arom.