Quantitative ultrasonic characterization of lesion composition and remodeling in atherosclerotic rabbit aorta.
Quantitative ultrasonic characterization of lesion composition and remodeling in atherosclerotic rabbit aorta.
复制标题
动脉粥样硬化兔主动脉病变成分和重塑的定量超声表征。
DOI：
10.1161/01.atv.13.10.1543
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Daugherty,A
中科院分区：
文献类型：
--
作者：
Wickline,SA;Shepard,RK;Daugherty,A
我们之前已经证明，高频率，高分辨率的超声波可以表征胆固醇喂养的兔子脂肪斑块的声学特性和组成。为了确定定量超声是否可以通过检测饮食改变引起的脂肪到纤维类型的变化来描述动脉粥样硬化病变的消退，我们给6只新西兰大白兔喂了3个月的2%胆固醇的饮食，然后再喂了3个月的标准饮食，以促进纤维内膜病变的发展。切除主动脉段，利用50 MHz的声学显微镜从每个标本的400至600个独立位置获得背散射射频数据。对照数据通过测量无病变主动脉邻近部位的后向散射提供。纤维内膜病变的组织学和免疫细胞化学分析证实了平滑肌细胞和大量结缔组织的存在，几乎没有明显的脂质。正常主动脉段的反向散射（-30.7 +/- 1.0 dB）大约是纤维病变（-42.4 +/- 1.0 dB, P < 0.05）的10倍。我们之前报道过脂肪性病变的综合后向散射为-50.6 +/- 0.7 dB，大约是纤维性病变的10倍（P < 0.05）。每种组织类型介质的综合后向散射值大致相等（正常组织、纤维组织和脂肪组织分别为-30.0 +/- 1.7 dB和-30.7 +/- 1.6 dB和-33.4 +/- 0.8 dB, P =无统计学意义）。（摘要删节250字）
We have previously shown that high-frequency, high-resolution ultrasound can characterize the acoustic properties and composition of fatty plaques in cholesterol-fed rabbits. To determine whether quantitative ultrasound can delineate the regression of atherosclerotic lesions by detecting a change in their composition from fatty to fibrous types induced by alterations in dietary regimen, we fed six New Zealand White rabbits a 2% cholesterol diet for 3 months, followed by a standard diet for 3 additional months to promote the development of fibrous intimal lesions. Segments of aortas were excised, and backscattered radiofrequency data were acquired from 400 to 600 independent sites in each specimen with an acoustic microscope operated at 50 MHz. Control data were provided by measuring backscatter from adjacent portions of the aortas devoid of lesions. Histological and immunocytochemical analyses of the fibrous intimal lesions confirmed the presence of smooth muscle cells and abundant connective tissue with little appreciable lipid. Backscatter from normal aortic segments (-30.7 +/- 1.0 dB) was approximately 10-fold greater than that from fibrous lesions (-42.4 +/- 1.0 dB; P < .05). We previously reported that integrated backscatter from fatty lesions was -50.6 +/- 0.7 dB, or approximately 10-fold less than that from fibrous lesions (P < .05). Values for integrated backscatter from the media of each tissue type were approximately equal (-30.0 +/- 1.7 versus -30.7 +/- 1.6 versus -33.4 +/- 0.8 dB for normal versus fibrous versus fatty tissues, respectively; P = not significant).(ABSTRACT TRUNCATED AT 250 WORDS)